Influence of KDEL on the fate of trimeric or assembly-defective phaseolin: selective use of an alternative route to vacuoles

The Plant Cell
L FrigerioA Vitale

Abstract

The tetrapeptide KDEL is commonly found at the C terminus of soluble proteins of the endoplasmic reticulum (ER), and it contributes to their localization by interacting with a receptor that recycles between the Golgi complex and the ER. We investigated the effects of the addition of KDEL to phaseolin, a protein normally delivered from the ER to storage vacuoles via the Golgi complex. We show that KDEL prevents acquisition of trans-Golgi-specific glycan modifications and causes interactions with the chaperone BiP that are distinct from the ones between BiP and defective proteins. KDEL markedly increases the stability of phaseolin, but a small proportion of phaseolin-KDEL slowly reaches the vacuole without undergoing Golgi-mediated glycan modifications, in a process that can be inhibited by brefeldin A but not monensin. Our results indicate that KDEL can operate with high efficiency before proteins can reach the late Golgi cisternae but allows or promotes delivery to vacuoles via an alternative mechanism. However, addition of KDEL does not alter the destiny of an assembly-defective form of phaseolin, suggesting that the plant ER quality control mechanism is dominant over KDEL effects.

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Citations

Mar 5, 2011·Plant Cell Reports·Amélie SaumonneauMathilde Francin-Allami
Sep 25, 2012·Plant Cell Reports·Mathilde Francin-AllamiYves Popineau
Oct 1, 2009·Plant Molecular Biology·M Lucrecia AlvarezGuy A Cardineau
Dec 12, 2001·Trends in Plant Science·C Timpte
Jun 10, 2008·Journal of Experimental Botany·Maddalena de VirgilioAlessandro Vitale
Oct 27, 2004·Plant Physiology·Davide MainieriAlessandro Vitale
Sep 11, 2001·The Plant Cell·B A PhillipsonJ Denecke
Feb 22, 2005·The New Phytologist·Chris Hawes
Aug 18, 2010·Plant Molecular Biology·Francesca De MarchisMichele Bellucci
Aug 24, 2005·The Journal of Cell Biology·Misoon ParkInhwan Hwang
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Kentaro TamuraIkuko Hara-Nishimura
Apr 9, 2008·Plant Biotechnology Journal·David J WangShengwu Ma
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Eva M KleinAlessandro Vitale

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