PMID: 40582Jan 1, 1979

Influence of L-tryptophan on the activity of tryptophan oxygenase in rat liver slices

Arzneimittel-Forschung
H KrögerL Charton

Abstract

Earlier experiments in vivo with adrenalectomized rats showed that for the induction of tryptophan oxygenase and tyrosine aminotransferase small doses of cortisone acetate and the corresponding substrate are necessary. We performed the similar experiments in vitro with liver slices from adrenalectomized rats which were pretreated with cortisone acetate in vivo. Studies with alpha-amanitine and cordycepin--given in vivo--showed, that the synthesis of m -RNA for tryptophan oxygenase after application of 1 mg/kg cortisone acetate lasted for less than 2 h. The results obtained in vitro were very similar to those reported earlier. Maximum activity is obtained in the presence of the substrate L-tryptophan. When adding other amino acids instead of the substrate no effect can be observed. The same is true also for actinomycin D. In the presence of puromycin and cycloheximide the enzyme activity decreases.

Related Concepts

Amanitins
Tryptophan
Amino Acids, I.V. solution additive
Tyrosine Aminotransferase
Puromycin Dihydrochloride
Alpha-Amanitin
Enzyme Activity
PMS-Tryptophan
Blood Enzyme Activity (Lab Test)
Adreson

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.