Influence of leucine infusion on intracellular amino acids in humans

European Journal of Clinical Investigation
A AlvestrandL S Eriksson

Abstract

A continuous intravenous infusion of L-leucine (300 mumols min-1) was given to 12 healthy females over a 2 1/2 h period. Arterial plasma concentrations of amino acids and the keto acids of the branched-chain amino acids (BCAA) were measured. In six subjects muscle biopsies were taken before and at the end of the infusion for determination of intracellular (i.c.) free amino acid concentrations, and leg exchange of amino acids was measured. During infusion the plasma level of leucine rose sixfold. Approximately 40% of the infused amount was taken up by muscle. Of this, half was accumulated intracellularly, where the free leucine concentration increased from basal 190 +/- 22 to 580 +/- 110 mumols l-1 ICW (intracellular water) at the end of infusion. The concentrations of most other amino acids, above all the other BCAA and the aromatic amino acids, decreased, by 17-48% in the i.c. pool and by 17-79% in plasma. The plasma level of ketoisocaproic acid (KIC), the keto acid of leucine, increased in parallel with that of leucine. The concentration of keto valine, ketoisovaleric acid (KIV), decreased by 75%, whereas the keto acid of isoleucine, ketomethylvaleric acid (KMV), was unchanged. Leg release of alanine decreased significantly, ...Continue Reading

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Citations

Aug 11, 2000·Journal of Gastroenterology and Hepatology·C PlatellJ C Hall
Jul 7, 2001·American Journal of Physiology. Endocrinology and Metabolism·E Blomstrand, B Saltin
Oct 21, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Matthijs K C HesselinkPatrick Schrauwen
Jul 15, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·William ApróEva Blomstrand
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Jan 31, 2014·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Marcus MobergEva Blomstrand
Dec 1, 2011·American Journal of Physiology. Endocrinology and Metabolism·Marcus BorgenvikEva Blomstrand
Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony M J SanchezRobin B Candau
Feb 23, 2019·Interventional Medicine & Applied Science·Imanipour VahidSadeghi Mehdi
Jun 3, 2005·The Journal of Nutrition·Dwight E Matthews
Dec 21, 2005·The Journal of Nutrition·Michael J RenniePaul Greenhaff
Dec 21, 2005·The Journal of Nutrition·Eva BlomstrandRickard Köhnke

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