PMID: 9550202Apr 29, 1998Paper

Influence of leukotrienes on in vitro growth of human mammary carcinoma cell line MCF-7

European Journal of Obstetrics, Gynecology, and Reproductive Biology
A PrzylipiakF M Köhn

Abstract

The aim of this work was to study the action of leukotrienes on the growth of human mammary cancer cells MCF-7. The growth of the cells was measured by incorporation of 3H-thymidine. The action of leukotriene (LT)B4, LTD4, LTC4, LTE4 or arachidonate (AA) was tested in human mammary cancer cells MCF-7 in vitro. LTB4 or LTD4 but not LTC4 or LTE4 reduced significant incorporation of 3H-thymidine in MCF-7 cells up to 52% or 56% respectively, when administered in concentrations 0.1-1000 pM. Agents in concentrations of 0.01 pM or 10000 pM did not effect 3H-thymidine incorporation. We have shown, that MCF-7 cells synthesise LTB4 when treated with calcium ionophor A23187 (10 microM). Leukotriene-antagonist LY171883 (10 microM) lifts inhibitory effects of LTB4 or LTD4. Arachidonic acid (10 microM) inhibits 3H-thymidine incorporation up to 72%. 5-lipoxygenase inhibitor MK-886 (100 nM) lifts the inhibitory effect of arachidonate. LTB4 or LTD4 inhibits MCF-7 breast cancer cell growth. LT-receptors mediate the growth-inhibitory effect of LTB4 or LTD4.

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Citations

Sep 16, 2011·Lipids in Health and Disease·Undurti N Das, N Madhavi
Oct 13, 2010·Biochemical and Biophysical Research Communications·Peng GaoJie Zheng
Jul 8, 2017·Angewandte Chemie·Christophe CopéretPavel A Zhizhko

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