Influence of levamisole and other angiogenesis inhibitors on angiogenesis and endothelial cell morphology in vitro

Cancers
Tina FriisGunnar Houen

Abstract

Angiogenesis, the formation of new blood vessels from existing vessels is required for many physiological processes and for growth of solid tumors. Initiated by hypoxia, angiogenesis involves binding of angiogenic factors to endothelial cell (EC) receptors and activation of cellular signaling, differentiation, migration, proliferation, interconnection and canalization of ECs, remodeling of the extracellular matrix and stabilization of newly formed vessels. Experimentally, these processes can be studied by several in vitro and in vivo assays focusing on different steps in the process. In vitro, ECs form networks of capillary-like tubes when propagated for three days in coculture with fibroblasts. The tube formation is dependent on vascular endothelial growth factor (VEGF) and omission of VEGF from the culture medium results in the formation of clusters of undifferentiated ECs. Addition of angiogenesis inhibitors to the coculture system disrupts endothelial network formation and influences EC morphology in two distinct ways. Treatment with antibodies to VEGF, soluble VEGF receptor, the VEGF receptor tyrosine kinase inhibitor SU5614, protein tyrosine phosphatase inhibitor (PTPI) IV or levamisole results in the formation of EC clus...Continue Reading

References

Feb 1, 1977·The Journal of Experimental Medicine·G Renoux, M Renoux
Jun 1, 1977·Cell·G Albrecht-Buehler
Feb 14, 1991·Biochemical and Biophysical Research Communications·M KusakaJ Folkman
Nov 1, 1991·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H C StevensonD R Parkinson
Jan 1, 1987·Immunology Letters·J M RedondoM Fresno
Feb 1, 1981·Immunopharmacology·I G OtternessM L Bliven
Aug 1, 1980·Drugs·M J Miller
Jul 1, 1995·The Journal of Cell Biology·D G ChalupowiczJ Martinez
Nov 1, 1995·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·A M SieuwertsJ A Foekens
Jun 1, 1996·Nature Medicine·M S O'ReillyJ Folkman
Jun 1, 1996·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·J B Hoying, S K Williams
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·N SinC M Crews
Dec 22, 1998·The Journal of Experimental Medicine·S E PikeG Tosato
Mar 2, 1999·Biochemical and Biophysical Research Communications·R RamchandranV P Sukhatme
Sep 24, 1999·Biochemical and Biophysical Research Communications·L TaddeiG Ramponi
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R YehC M Crews
Jan 5, 2001·British Journal of Pharmacology·M ArtwohlS M Baumgartner-Parzer
Mar 14, 2001·Journal of Neuro-oncology·R H GoldbrunnerJ C Tonn
Mar 27, 2001·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·G CaiD A Beacham
Dec 12, 2001·Psychotherapy and Psychosomatics·Valérie A G VentureyraChantal De Mey-Guillard
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rok HumarEdouard J Battegay
Sep 13, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tina FriisGunnar Houen
Oct 1, 2003·Angiogenesis·E T BishopD N Wheatley
Feb 26, 2004·Nature Medicine·Ronit Satchi-FainaroJudah Folkman
Aug 11, 2004·Angiogenesis·Jurjees HasanG C Jayson
Jan 7, 2005·Journal of Biomolecular Screening·Vladimir MastyuginFrank Buxton
Mar 16, 2005·Cancer Cell·Ronit Satchi-FainaroJudah Folkman

❮ Previous
Next ❯

Citations

Nov 18, 2018·Current Protein & Peptide Science·Gunnar Houen
Feb 20, 2016·Oncology Letters·Jun Shi, Pin-Kang Wei
Aug 21, 2018·Cytotechnology·Gönül UlusN Ülkü Karabay Yavaşoğlu
Dec 23, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gang WangMao Luo

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
nuclear translocation
flourescence-activated cell sorting
ELISA
xenografts
biopsy

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.