Influence of low density lipoproteins on cytosolic free Ca2+ concentration and dense tubular system in human platelets

Thrombosis Research
B ZhaoS Frye

Abstract

The cytosolic free Ca2+ concentration [Ca2+]i and dense tubular system (DTS) of washed human platelets were affected by low density lipoproteins (LDL) of 25 micrograms/ml at 37 degrees C for 10 minutes. After the incubation with LDL, the [Ca2+]i increased from 115 +/- 29 nM to 141 +/- 24 nM. LDL promoted the increase of [Ca2+]i (471 +/- 31 nM) induced by thrombin (0.03 U/ml) as compared to that which thrombin did alone (240 +/- 11 nM) (p < 0.05). The increased Ca2+ influx from the extracellular space is thought to be the reason of the increase in [Ca2+]i, since the effect of LDL was abolished by removal of external Ca2+ by EGTA. The DTS changed primarily from thin elongated forms to rounded vesicles. No evidence was noticed that LDL caused a mobilization of Ca2+ from the DTS.

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Citations

Oct 1, 1995·Biulleten' eksperimental'noĭ biologii i meditsiny·I I Vlasova, O A Azizova
Jan 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·Yashika GuptaD K Srivastava
Oct 20, 1998·Prostaglandins & Other Lipid Mediators·M M Mahfouz, F A Kummerow
Aug 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·A WeidtmannW Siess

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