Influence of magnesium ions on biofilm formation by Pseudomonas fluorescens

Microbiological Research
Bo Song, Laura G Leff

Abstract

Mg(2+) can potentially influence bacterial adhesion directly through effects on electrostatic interactions and indirectly by affecting physiology-dependent attachment processes. However, the effects of Mg(2+) on biofilm structure are largely unknown. In this study, Pseudomonas fluorescens was used to investigate the influence of Mg(2+) concentration (0, 0.1 and 1.0mM MgCl(2)) on biofilm growth. Planktonic and attached cells were enumerated (based on DAPI staining) while biofilm structures were examined via confocal laser scanning microscopy and three-dimensional structures were reconstructed. Mg(2+) concentration had no influence on growth of planktonic cells but, during biofilm formation, Mg(2+) increased the abundance of attached cells. For attached cells, the influence of Mg(2+) concentration changed over time, suggesting that the role of Mg(2+) in bacterial attachment is complex and dynamic. Biofilm structures were heterogeneous and surface colonization and depth increased with increasing Mg(2+) concentrations. Overall, for P. fluorescens, Mg(2+) increased initial attachment and altered subsequent biofilm formation and structure.

References

Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Jun 30, 2001·FEMS Microbiology Letters·S MerinoJ M Tomás
Jan 1, 1997·Water Science and Technology : a Journal of the International Association on Water Pollution Research·D W Koenig, D L Pierson
Sep 13, 2002·Applied Microbiology and Biotechnology·X Chen, P S Stewart
Nov 6, 2003·International Journal of Biological Macromolecules·Daniel LattnerChristian Mayer
May 11, 2005·Microbiological Research·Bo Song, Laura G Leff
Dec 1, 1998·Journal of Applied Microbiology·P StoodleyH M Lappin-Scott

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Citations

Oct 9, 2007·Analytical and Bioanalytical Chemistry·Ivana StanimirovaJürgen W Einax
Mar 26, 2010·Biofouling·C S SrinandanA S Nerurkar
Mar 17, 2009·Applied and Environmental Microbiology·Barry HeffernanEoin Casey
Jan 27, 2009·Applied and Environmental Microbiology·Claire PerrinCorinne Dorel
Feb 8, 2014·Applied Microbiology and Biotechnology·Virginia CarvalhaisNuno Cerca
Jan 8, 2014·Canadian Journal of Microbiology·Gang ZhouWen-Feng Hu
Jul 9, 2013·Colloids and Surfaces. B, Biointerfaces·Li Juan ZhongXiao Dong Chen
Jun 9, 2016·Journal of Environmental Sciences (China)·Wen HaoJianrong Zhu
Jan 5, 2011·Acta Biomaterialia·Kateryna BazakaElena P Ivanova
Mar 17, 2015·Current Opinion in Biotechnology·Kun GuoKorneel Rabaey
Feb 13, 2015·Applied Microbiology and Biotechnology·Virginia CarvalhaisRui Vitorino
May 7, 2016·PloS One·Mareike Klinger-StrobelOliwia Makarewicz
Nov 2, 2016·Molecular & Cellular Proteomics : MCP·Takfarinas KentacheJulie Hardouin
Aug 17, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Junghyun KimSeok Chung
May 19, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·E Rava, E Chirwa
Jun 17, 2015·PLoS Pathogens·Claudine BelonAnne-Béatrice Blanc-Potard
Jan 9, 2019·Frontiers in Microbiology·Sabrina VölkelFelicitas Pfeifer
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Qiyu GaoHongyu Li

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