Influence of magnesium sulfate on HCO3/Cl transmembrane exchange rate in human erythrocytes

Journal of Theoretical Biology
Ekaterina S ChernyshovaAndrei V Chernyshev

Abstract

Magnesium sulfate (MgSO4) is widely used in medicine but molecular mechanisms of its protection through influence on erythrocytes are not fully understood and are considerably controversial. Using scanning flow cytometry, in this work for the first time we observed experimentally (both in situ and in vitro) a significant increase of HCO3(-)/Cl(-) transmembrane exchange rate of human erythrocytes in the presence of MgSO4 in blood. For a quantitative analysis of the obtained experimental data, we introduced and verified a molecular kinetic model, which describes activation of major anion exchanger Band 3 (or AE1) by its complexation with free intracellular Mg(2+) (taking into account Mg(2+) membrane transport and intracellular buffering). Fitting the model to our in vitro experimental data, we observed a good correspondence between theoretical and experimental kinetic curves that allowed us to evaluate the model parameters and to estimate for the first time the association constant of Mg(2+) with Band 3 as KB~0.07mM, which is in agreement with known values of the apparent Mg(2+) dissociation constant (from 0.01 to 0.1mM) that reflects experiments on enrichment of Mg(2+) at the inner erythrocyte membrane (Gunther, 2007). Results o...Continue Reading

References

Nov 11, 1991·Journal of Inorganic Biochemistry·J L BockC S Springer
Jan 1, 1991·Annual Review of Physiology·P W Flatman
Jan 1, 1994·The American Journal of Medicine·R M McLean
Sep 1, 1996·European Journal of Biochemistry·A M BrunatiV Moret
Mar 1, 1997·Anesthesiology·S A LoerJ Tarnow
Apr 9, 1998·British Journal of Obstetrics and Gynaecology·T O Idama, S W Lindow
Jun 30, 1998·Pflügers Archiv : European journal of physiology·P MercirisF Giraud
Jul 1, 1998·The Journal of Cell Biology·M PucéatG Vassort
Oct 8, 1998·British Journal of Obstetrics and Gynaecology·M Luckas, I Aird
Sep 1, 1999·European Journal of Biochemistry·J E RaftosP W Flatman
Jan 5, 2000·British Journal of Anaesthesia·W J FawcettD A Male
May 11, 2000·Blood Reviews·J Poole
Jul 11, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S BeydemirO I Küfrevioglu
May 2, 2002·Clinical Biochemistry·Ludmila ZylinskaMiroslaw Soszynski
Jun 18, 2002·American Journal of Obstetrics and Gynecology·Robert MittendorfPaul G Tomich
Nov 8, 2002·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·A ElimianJ G Quirk
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Roy E Weber, Wolfgang Voelter
Apr 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roy E WeberPhilip S Low
Jun 30, 2004·Journal of Perinatology : Official Journal of the California Perinatal Association·Robert MittendorfPeter G Pryde
Jan 12, 2005·Clinical Biochemistry·Ana Carolina ArizaAli Halhali
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Aurelie ChansonYves Rayssiguier
Apr 12, 2005·Clinical Biochemistry·Janusz SzemrajLudmila Zylinska
Jul 9, 2005·Chest·Maurice BlitzBrian H Rowe
Feb 17, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ewa GulczynskaLudmila Zylinska
Dec 18, 2007·Journal of Theoretical Biology·Andrey V ChernyshevValeri P Maltsev
Feb 29, 2008·Applied Optics·A N ShvalovV P Maltsev
Feb 13, 2009·Stroke; a Journal of Cerebral Circulation·Anna G Euser, Marilyn J Cipolla
May 5, 2009·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Cilia AbadReinaldo Marín
Oct 22, 2010·The Journal of Physiology·Pawel SwietachRichard D Vaughan-Jones
Dec 3, 2010·The Journal of Nutrition, Health & Aging·Z UlgerO Akyol
May 7, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Dmitry I StrokotovValeri P Maltsev
May 26, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Johannes J M L HoffmannJoyce Curvers

❮ Previous
Next ❯

Citations

Nov 22, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ekaterina S YastrebovaAndrei V Chernyshev
Dec 19, 2016·Pflügers Archiv : European journal of physiology·Rossana MorabitoAngela Marino
Jun 19, 2020·Journal of Diabetes and Metabolic Disorders·Leonardo Parreira Silva NascimentoOdair Aguiar Junior

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.