Influence of mechanical hemolysis of blood on two D-dimer immunoassays

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
G LippiLuigi Ippolito

Abstract

Although there is broad information about the influence of spurious hemolysis on several laboratory tests, less is known on the bias produced on D-dimer testing. Four different pools were obtained from primary blood tubes, and each of them was divided into four aliquots. The first nonhemolyzed was centrifuged, the plasma was separated and then tested for hemolysis index and D-dimer. The second (hemolyzed aliquot A), third (hemolyzed aliquot B) and fourth (hemolyzed aliquot C) aliquots were mechanically hemolyzed by aspirating whole blood one, two and three times through a fine needle. The plasma was then separated and tested for hemolysis index and D-dimer. D-dimer was quantified by HemosIL AcuStar D-dimer and HemosIL D-dimer HS for ACL TOP. Undetectable hemolysis was present in aliquot nonhemolyzed (<0.5 g/l), whereas the concentration of cell-free hemoglobin significantly increased from hemolyzed aliquot A (5.5-7.0 g/l hemoglobin) to hemolyzed aliquot B (11.5 and 15.0 g/l hemoglobin) and hemolyzed aliquot C (20-22 g/l hemoglobin). The plasma concentration of D-dimer decreased from aliquots nonhemolyzed to hemolyzed aliquot C, achieving clinical significance fromhemolyzed aliquot A and hemolyzed aliquot B when measured with D-...Continue Reading

References

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Nov 13, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Gian L SalvagnoGian C Guidi
Jun 25, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiMario Plebani
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Oct 1, 2010·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·David Green
Jan 26, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiRossana Fontana
Aug 31, 2011·Critical Reviews in Clinical Laboratory Sciences·Giuseppe LippiGianfranco Cervellin
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Jan 10, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Giuseppe LippiGian C Guidi

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Citations

Nov 21, 2013·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Giuseppe LippiEmmanuel J Favaloro
Feb 13, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Edward Ki Yun LeungKiang-Teck J Yeo
Jun 17, 2016·International Journal of Laboratory Hematology·A WoolleyS Kitchen
Mar 20, 2020·International Journal of Laboratory Hematology·Ylva HedelandPeter Ridefelt
Oct 25, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe LippiArmando Tripodi
Jan 30, 2019·Critical Reviews in Clinical Laboratory Sciences·Julien FavresseFrançois Mullier

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