Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids.

Applied and Environmental Microbiology
K C ThomasW M Ingledew

Abstract

Acetic acid (167 mM) and lactic acid (548 mM) completely inhibited growth of Saccharomyces cerevisiae both in minimal medium and in media which contained supplements, such as yeast extract, corn steep powder, or a mixture of amino acids. However, the yeast grew when the pH of the medium containing acetic acid or lactic acid was adjusted to 4.5, even though the medium still contained the undissociated form of either acid at a concentration of 102 mM. The results indicated that the buffer pair formed when the pH was adjusted to 4.5 stabilized the pH of the medium by sequestering protons and by lessening the negative impact of the pH drop on yeast growth, and it also decreased the difference between the extracellular and intracellular pH values (Delta(pH)), the driving force for the intracellular accumulation of acid. Increasing the undissociated acetic acid concentration at pH 4.5 to 163 mM by raising the concentration of the total acid to 267 mM did not increase inhibition. It is suggested that this may be the direct result of decreased acidification of the cytosol because of the intracellular buffering by the buffer pair formed from the acid already accumulated. At a concentration of 102 mM undissociated acetic acid, the yeast ...Continue Reading

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Citations

Mar 24, 2010·Applied Microbiology and Biotechnology·Bing-Zhi Li, Ying-Jin Yuan
Sep 1, 2004·Journal of Industrial Microbiology & Biotechnology·Kelly A Skinner, Timothy D Leathers
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·Tara GravesRonan Power
Oct 8, 2008·Journal of Industrial Microbiology & Biotechnology·Renyong ZhaoDonghai Wang
Dec 13, 2006·Biotechnology Letters·Kelly A Skinner-NemecTimothy D Leathers
Jun 28, 2011·Applied Biochemistry and Biotechnology·Cheng-Ming ZhangLei Tang
Jan 21, 2011·BMC Microbiology·Tatyana A SemenovaMorten Schiøtt
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Jul 16, 2013·Journal of Bioscience and Bioengineering·Masataka TomitakaTakashi Akamatsu
Oct 1, 2015·Applied Biochemistry and Biotechnology·Sarah A LeeElliot Altman
Feb 20, 2013·Journal of Bioscience and Bioengineering·Makoto WakamatsuTakashi Akamatsu
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