Influence of Menopause on Inflammatory Cytokines during Murine and Human Bone Fracture Healing

International Journal of Molecular Sciences
Verena FischerMelanie Haffner-Luntzer

Abstract

Postmenopausal females display a chronic inflammatory phenotype with higher levels of circulating pro-inflammatory cytokines. Furthermore, the inflammatory response to injury may be altered under estrogen-deficiency, because it was shown previously that estrogen-deficient mice displayed increased levels of the inflammatory cytokines Midkine (Mdk) and Interleukin-6 (IL-6) in the early fracture hematoma. Because a balanced immune response to fracture is required for successful bone regeneration, this might contribute to the delayed fracture healing frequently observed in osteoporotic, postmenopausal fracture patients. In this study, we aimed to investigate whether further cytokines in addition to Mdk and IL-6 might be affected by estrogen-deficiency after fracture in mice and whether these cytokines are also relevant during human fracture healing. Additionally, we aimed to investigate whether serum from male vs. female fracture patients affects osteogenic differentiation of human mesenchymal stem cells (MSCs). To address these questions, female mice were either sham-operated or ovariectomized (OVX) and subjected to standardized femur osteotomy. A broad panel of pro- and anti-inflammatory cytokines was determined systemically and ...Continue Reading

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Citations

Aug 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Melanie Haffner-LuntzerMichael Lehmicke
Nov 30, 2019·Current Pharmaceutical Design·Su-Kang ShanLing-Qing Yuan
Dec 6, 2019·Oncogene·Panagiota S FilippouAnastasia Constantinidou
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Aug 15, 2020·Bone & Joint Research·Simon K-H ChowWing-Hoi Cheung
Jan 17, 2021·International Journal of Molecular Sciences·Birte WeberMiriam Kalbitz
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Lena SteppeMelanie Haffner-Luntzer
Feb 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaokang JiaShuisheng Wu
May 25, 2021·Seminars in Cell & Developmental Biology·Verena Fischer, Melanie Haffner-Luntzer
Aug 27, 2021·Frontiers in Physiology·Melanie Haffner-LuntzerAnita Ignatius
Oct 12, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Verena FischerAnita Ignatius

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Protein Assay
ELISA
PCR

Software Mentioned

GrapdPad
GraphPad Prism
LinRegPCR

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