Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids

Indian Journal of Microbiology
Sanjay K S PatelJung-Kul Lee

Abstract

Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of β-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temperature stability compared to free form. Thermal stability of hybrid improved up to 26-fold at 60 °C. After 10 cycles of reuse, immobilized enzyme retained 93.8% of residual activity. These results suggested that metal ions played a significant role in the enzyme immobilization as a protein-inorganic hybrid. Overall, this strategy can be potentially applied to enhance the properties of enzymes though effective encapsulation for the broad biotechnological applications.

References

Jun 5, 2012·Nature Nanotechnology·Jun GeRichard N Zare
Feb 11, 2014·Journal of Microbiology and Biotechnology·Sanjay K S PatelJung Kul Lee
Dec 23, 2016·ACS Applied Materials & Interfaces·Sanjay K S PatelJung-Kul Lee
Nov 12, 2017·Scientific Reports·Muhammad Zahid AnwarJung-Kul Lee
Feb 1, 2018·Journal of Microbiology and Biotechnology·Ashok KumarJung-Kul Lee
Feb 13, 2018·Indian Journal of Microbiology·Ashok KumarJung-Kul Lee
Aug 11, 2018·Bioresource Technology·José Carlos Santos SalgadoRichard John Ward
Aug 12, 2018·International Journal of Biological Macromolecules·Thamara C CoutinhoCristiane S Farinas
Feb 8, 2019·Indian Journal of Microbiology·Sachin V OtariJung-Kul Lee

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Citations

Jul 14, 2020·Indian Journal of Microbiology·Brian N MathibeBrett I Pletschke
Aug 8, 2019·Indian Journal of Microbiology·Smitha Mony SreedharanRajni Singh
Nov 26, 2019·Indian Journal of Microbiology·Hemant J Purohit
Oct 23, 2020·Indian Journal of Microbiology·Bhawna SharmaPradeep Kumar

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Methods Mentioned

BETA
scanning electron microscopy
scanning
confocal microscopy

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