Influence of Microenvironment on Mesenchymal Stem Cell Therapeutic Potency: From Planar Culture to Microcarriers

Frontiers in Bioengineering and Biotechnology
Ang-Chen TsaiYan Li

Abstract

Human mesenchymal stem cells (hMSCs) are a promising candidate in cell therapy as they exhibit multilineage differentiation, homing to the site of injury, and secretion of trophic factors that facilitate tissue healing and/or modulate immune response. As a result, hMSC-derived products have attracted growing interests in preclinical and clinical studies. The development of hMSC culture platforms for large-scale biomanufacturing is necessary to meet the requirements for late-phase clinical trials and future commercialization. Microcarriers in stirred-tank bioreactors have been widely utilized in large-scale expansion of hMSCs for translational applications because of a high surface-to-volume ratio compared to conventional 2D planar culture. However, recent studies have demonstrated that microcarrier-expanded hMSCs differ from dish- or flask-expanded cells in size, morphology, proliferation, viability, surface markers, gene expression, differentiation potential, and secretome profile which may lead to altered therapeutic potency. Therefore, understanding the bioprocessing parameters that influence hMSC therapeutic efficacy is essential for the optimization of microcarrier-based bioreactor system to maximize hMSC quantity without ...Continue Reading

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Citations

Nov 26, 2020·Scientific Reports·Brent M BijonowskiSamuel C Grant
Jan 16, 2021·ACS Biomaterials Science & Engineering·John D KruttyPadma Gopalan
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Asim Cengiz AkbulutLeon J Schurgers
Mar 4, 2021·American Journal of Physiology. Cell Physiology·Dimitrios I Zeugolis
Mar 19, 2021·Critical Reviews in Biotechnology·Alonso Ornelas-GonzálezMarco Rito-Palomares
Apr 4, 2021·International Journal of Molecular Sciences·Silvia Fernández-FrancosFrancisco J Vizoso
May 1, 2021·Advanced Healthcare Materials·Gilad Doron, Johnna S Temenoff
Aug 6, 2021·Frontiers in Bioengineering and Biotechnology·Qize XuanPing Wang

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Methods Mentioned

BETA
microprinting
atomic force microscopy

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