Influence of Molecular Structure on O2-Binding Properties and Blood Circulation of Hemoglobin‒Albumin Clusters

PloS One
Kana YamadaTeruyuki Komatsu

Abstract

A hemoglobin wrapped covalently by three human serum albumins, a Hb-HSA3 cluster, is an artificial O2-carrier with the potential to function as a red blood cell substitute. This paper describes the synthesis and O2-binding properties of new hemoglobin‒albumin clusters (i) bearing four HSA units at the periphery (Hb-HSA4, large-size variant) and (ii) containing an intramolecularly crosslinked Hb in the center (XLHb-HSA3, high O2-affinity variant). Dynamic light scattering measurements revealed that the Hb-HSA4 diameter is greater than that of either Hb-HSA3 or XLHb-HSA3. The XLHb-HSA3 showed moderately high O2-affinity compared to the others because of the chemical linkage between the Cys-93(β) residues in Hb. Furthermore, the blood circulation behavior of 125I-labeled clusters was investigated by assay of blood retention and tissue distribution after intravenous administration into anesthetized rats. The XLHb-HSA3 was metabolized faster than Hb-HSA3 and Hb-HSA4. Results suggest that the molecular structure of the protein cluster is a factor that can influence in vivo circulation behavior.

References

Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Nov 1, 1996·World Journal of Surgery·S A Gould, G S Moss
Jun 20, 1998·The Journal of Biological Chemistry·R J RohlfsR M Winslow
Mar 29, 2003·Transfusion·Kim D VandegriffRobert M Winslow
Apr 19, 2003·Journal of Internal Medicine·R M Winslow
Jun 6, 2003·Journal of the American Chemical Society·Ronald Kluger, Jie Zhang
Sep 20, 2005·Journal of Molecular Biology·Jamie GhumanStephen Curry
Apr 30, 2008·JAMA : the Journal of the American Medical Association·Charles NatansonSidney M Wolfe
Aug 13, 2008·Expert Opinion on Biological Therapy·Jonathan S JahrJennifer C Lim
Oct 18, 2008·Bioconjugate Chemistry·Yalong ZhangAndre F Palmer
Oct 30, 2008·Biochemistry·Dongxin Hu, Ronald Kluger
Apr 16, 2010·Current Opinion in Chemical Biology·Ronald Kluger
Jul 30, 2015·Scientific Reports·Risa HarukiTeruyuki Komatsu
Aug 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Takuya KimuraTeruyuki Komatsu

❮ Previous
Next ❯

Citations

Mar 25, 2017·Journal of Functional Biomaterials·Kazuaki TaguchiMasaki Otagiri
Jul 25, 2021·The American Journal of the Medical Sciences·Caiyun FengHaiyan Xiong

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
gel filtration
size-exclusion chromatography
protein assay
dynamic light scattering
chemical
transmission electron microscopy

Software Mentioned

Excel
BMTEG

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.