Influence of muscle cell substrates on differentiation of intrafusal fiber types in neonatal rats

Neuroscience
J KuceraY Gao

Abstract

Rat muscle spindles contain one nuclear bag2, one nuclear bag1, and two nuclear chain fibers. The three different types of intrafusal fiber in spindles may be a reflection of concomitant changes in proportions of slow primary, slow/fast secondary, and fast secondary myotubes during the period of spindle development. We examined whether experimentally altering the available muscle substrates would impact the intrafusal fiber type composition of spindles. De novo formation of spindles in muscles devoid of primary myotubes was induced by crushing the nerve to the medial gastrocnemius muscle in newborn rats and administering nerve growth factor for ten days afterwards. Encapsulated fibers of the reinnervated muscles examined one month after nerve crush had myofibrillar adenosine triphosphatase and myosin heavy chain profiles similar to normal bag2, bag1, or chain intrafusal fibers. However, spindles in reinnervated muscles contained fewer fibers than controls. Most experimental spindles contained chain and/or bag1 fibers, the two fiber types which ordinarily arise during secondary myogenesis. In contrast, bag2 fibers, fibers that normally form concomitant with primary myogenesis, were absent from nearly 90% of spindles in reinnerva...Continue Reading

References

Nov 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J KuceraW K Engel
Feb 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J KuceraL Gorza
Mar 2, 1992·Neuroscience Letters·J Kucera, J Walro
Apr 1, 1990·Developmental Biology·K CondonW J Thompson
Jan 1, 1990·Histochemistry·J Kucera, J M Walro
Oct 23, 1989·Neuroscience Letters·J Kucera, J Walro
Oct 1, 1989·The American Journal of Anatomy·J KuceraJ Reichler
Aug 1, 1989·Journal of Muscle Research and Cell Motility·J F Hoh, S Hughes
Jan 1, 1989·Experimental Brain Research·J PalecekP Hník
Oct 1, 1970·Archives of Neurology·M H Brooke, K K Kaiser
Dec 1, 1982·The Journal of Cell Biology·D BaderD A Fischman

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