Influence of myosin isoforms on tension cost and crossbridge kinetics in skinned rat cardiac muscle

Clinical and Experimental Pharmacology & Physiology
G H RossmanithJ F Hoh

Abstract

1. In attempting to consolidate the role of ventricular isomyosins in regulating the contractility of the myocardium, actomyosin ATPase and crossbridge kinetics were obtained at 24 degrees C in chemically skinned isometrically contracting cardiac muscles containing V1 and V3 isomyosins. 2. The ATPase activity was measured at various levels of Ca2+ activation by the enzymatic coupling of ATP hydrolysis with the conversion of NADH to NAD+. The crossbridge kinetics were inferred from small-amplitude perturbations of muscle length and muscle tension, and characterized by the frequency-domain parameter fmin. 3. The ATPase rates of V1 and V3 muscles obtained at various levels of Ca2+ activation were plotted against the corresponding proportional tensions. The ATPase vs tension plots were linear with slopes of 4.92 nmol/min-1 per mm per mN and 1.98 nmol/min-1 per mm per mN, respectively for, V1 and V3 muscles. Individual calculations of ATPase-to-tension ratios (nmol/min-1 per mm per mN) gave corresponding averages of 4.98 +/- 0.12 (s.e.m., n = 12) and 2.16 +/- 0.12 (s.e.m., n = 10). The myosin isoform induced proportional change in tension cost was accompanied by a similar change in fmin (4.1 +/- 0.1 Hz and 1.95 +/- 0.03 Hz, means +/...Continue Reading

References

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Citations

Sep 22, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Joseph F Y HohWendy W H Zhong
Jun 2, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yoonah KimJoseph F Y Hoh
Nov 3, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Joseph F Y HohWendy W H Zhong
Jul 23, 1998·Clinical and Experimental Pharmacology & Physiology·G H Rossmanith, O B Tjokorda
Jan 11, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Matthew R LocherRichard L Moss
Apr 28, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Matthew R LocherRichard L Moss
Mar 6, 2008·Journal of Muscle Research and Cell Motility·Joseph F Y HohGunther H Rossmanith

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