PMID: 9425120Feb 28, 1998Paper

Influence of N-terminal sequence variation on the sorting of major adenylate kinase to the mitochondrial intermembrane space in yeast

The Biochemical Journal
Wolfhard BandlowR Schricker

Abstract

Major adenylate kinase (Aky2p) from yeast has no cleavable presequence and occurs in identical form in the mitochondrial intermembrane space (6-8%) and in the cytoplasm (approx. 90%). To identify the signal(s) on Aky2p that might be required for mitochondrial import, the N-terminal region was examined. The N-terminus of Aky2p can guide at least two cytoplasmic passengers, dihydrofolate reductase from mouse and UMP kinase (Ura6p) from yeast, to the intermembrane space in vivo, showing that the N-terminus harbours import information. In contrast, deletion of the eight N-terminal amino acid residues or the introduction of two compensating frameshifts into this segment does not abolish translocation into the organelle's intermembrane space. Thus internal targeting and sorting information must be present in Aky2p as well. Neither a pronounced amphiphilic alpha-helical moment nor positive charges in the N-terminal region is a necessary prerequisite for Aky2p to reach the intermembrane space. Even a surplus of negative charges in mutant N-termini does not impede basal import into the correct submitochondrial compartment. The potential to form an amphipathic alpha-helical structure of five to eight residues close to the N-terminus sign...Continue Reading

Citations

Sep 25, 1998·The Journal of Biological Chemistry·C KnoxO Pines
Jun 5, 2002·The Journal of Biological Chemistry·Roland SchrickerWolfhard Bandlow
Jan 1, 2014·Frontiers in Molecular Biosciences·Bella Kalderon, Ophry Pines
Jul 11, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Neta Regev-Rudzki, Ophry Pines
Nov 26, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David J McKinnonGordon R Gray
Jul 16, 2010·Expert Opinion on Drug Metabolism & Toxicology·Michelle C SangarNarayan G Avadhani
Aug 12, 2014·Biochemical Society Transactions·Carmelina Petrungaro, Jan Riemer
Sep 25, 2002·The Journal of Biological Chemistry·Tapan K Biswas, Godfrey S Getz
Nov 19, 2003·European Journal of Biochemistry·Héctor VillaDavid Ordóñez
Jul 20, 2010·Biochimica Et Biophysica Acta·Ohad Yogev, Ophry Pines

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