Influence of oestradiol-17 beta on interleukin-1 secretion by endotoxin-stimulated porcine mononuclear cells in vitro

Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B
U Magnusson, M Villacres-Eriksson

Abstract

A bioassay for porcine interleukin-1 (IL-1) was validated. By use of this bioassay, the influence of increasing concentrations of oestradiol-17 beta on IL-1 secretion by endotoxin-stimulated porcine mononuclear cells (MNC) in vitro was studied. Supernatants from cultures of porcine MNC stimulated with LPS (0.1-10 micrograms/ml) induced a proliferative response in the IL-1 and IL-2 dependent cell line D10.G4.1 (D-10) in a dose-dependent manner with respect to LPS. Supernatants of LPS stimulated porcine MNC did not show any IL-2 activity in an bioassay for IL-2. Porcine MNC were cultured in the presence of increasing concentrations of oestradiol-17 beta (42-1500 pmol/l) and stimulated with LPS (10 micrograms/ml) or in the absence of oestradiol and LPS. The IL-1 secretion by the MNC, as determined by the D-10 bioassay, was significantly (P < 0.01) suppressed by the highest oestradiol concentration tested (1500 pmol/l). The results indicate that high physiological concentrations of oestradiol-17 beta may affect the secretion of IL-1 by porcine MNC.

References

May 1, 1992·Research in Veterinary Science·F P Winstanley, P D Eckersall
Feb 1, 1991·Veterinary Immunology and Immunopathology·I Edfors-LiljaC Fossum
Oct 1, 1990·American Journal of Reproductive Immunology : AJRI·H MoriT Tamaya
Feb 1, 1990·Veterinary Immunology and Immunopathology·S A MayP Lees
Jun 21, 1989·Journal of Immunological Methods·S J Hopkins, M Humphreys
Oct 1, 1988·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·U Magnusson, C Fossum
Jun 1, 1988·European Journal of Immunology·M HelleL A Aarden
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E A Kurt-JonesE R Unanue

❮ Previous
Next ❯

Citations

Jul 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H Holst, U Magnusson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.