PMID: 22570923May 11, 2012Paper

Influence of orthodontic treatment on oral streptococci

Roumanian Archives of Microbiology and Immunology
Theodor-Cristian VizitiuEcaterina Ionescu

Abstract

Objective of this study is to evaluate the changes of the oral microbial flora, concentrating on the oral streptococci, after the first 3 and 6 months of orthodontic treatment. 40 patients, aged 7-17, that presented for orthodontic treatment between April and September 2010 in the Department of Orthodontics and Dento-Facial Orthopedics of "Carol Davila" University of Medicine and Pharmacy, Bucharest have been selected. According to the protocol, coronary and subgingival plaque was collected from the dental surface before starting any orthodontic treatment (T0), 3 months after wearing orthodontic appliances (T1) and 6 months after wearing orthodontic appliances (T2). The samples were studied in Cantacuzino National Institute of Research-Development for Microbiology and Immunology [isolation on Columbia agar with 5% sheep blood, identification on morphotinctorial, growth and biochemical characteristics using API 20 STREP (BioMerieux)]. Bacterial concentration (colony-forming units/sample = CFU/sample) for the aerobic and anaerobic flora was calculated by the serial dilution method of counting bacteria. 106 strains of oral streptococci were isolated from dental plaque, belonging to 6 species (Streptococcus mitis, Streptococcus ora...Continue Reading

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