Influence of ouabain and dihydroouabain on the circular dichroism of cardiac plasmalemmal microsomes.

Naunyn-Schmiedeberg's Archives of Pharmacology
H LüllmannT Rüther

Abstract

1. The influence of ouabain on the tertiary structure of cardiac plasmalemmal proteins was investigated by means of circular dichroism measurement. Purified plasmalemmal microsomes were obtained by sucrose gradient centrifugation. The CD-spectra of the membranal proteins were shifted to the red and the amplitudes were smaller than those of the same proteins after solubilization. 2. Ouabain induced an increase of the ellipticity bands at 210 and 222 nm of about 50% above the level yielded with microsomes after sonication. At 222 nm ouabain exhibited the half maximum effect at a concentration of 5 X 10(-9) M. The effect could, however, only be exerted if the inside of the microsomes was exposed to ouabain by sonication, thus reflecting the inside-out nature of the plasmalemmal microsomes. 3. The high specificity of the ouabain effect was underlined by the following experiments: a) Dihydroouabain, a much less cardioactive derivative of ouabain proved to be ineffective in corresponding concentrations, b) ouabain had no influence upon the CD spectrum of microsomes derived from cardiac sarcoplasmic reticulum, c) a detergent-like action of ouabain underlying the observed effect can be excluded since highly active tensides, i.e. desoxy...Continue Reading

References

Nov 22, 1991·Proceedings. Biological Sciences·R D KeynesJ M Bekkers
Dec 1, 1974·Journal of Molecular and Cellular Cardiology·G SaccomaniG Sachs
Apr 3, 1973·Biochimica Et Biophysica Acta·R Coleman
Aug 9, 1973·Biochimica Et Biophysica Acta·W R RedwoodT E Thompson
Jan 1, 1974·Advances in Cardiology·H Lüllmann, T Peters
Sep 10, 1973·Biochimica Et Biophysica Acta·R F ZwaalC M Colley
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B P Dorman, M F Maestre
Feb 1, 1973·Journal of Molecular and Cellular Cardiology·W G Nayler
Jan 1, 1973·Methods in Enzymology·A S Schneider
Jan 1, 1973·Methods in Enzymology·A J AdlerG D Fasman
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D W Urry, J Krivacic
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M GlaserS I Chan
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A S SchneiderK Rosenheck
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D J Gordon, G Holzwarth
Mar 1, 1970·Archives of Biochemistry and Biophysics·D W UrryL Masotti
May 23, 1970·Nature·C van Breemen, P De Weer
Apr 1, 1971·British Journal of Pharmacology·K D KolendaT Peters
Feb 15, 1964·Klinische Wochenschrift·K REPKE
Dec 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J Lenard, S J Singer

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Citations

Mar 24, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·R RösenA Stier
Oct 12, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·C AlsenR W Sattler
Mar 31, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·T Peter
Jul 1, 1984·Progress in Cardiovascular Diseases·T W SmithJ D Marsh
May 1, 1984·Progress in Cardiovascular Diseases·T W SmithJ D Marsh
Mar 1, 1984·Progress in Cardiovascular Diseases·T W SmithJ D Marsh
Nov 1, 1980·Circulation Research·N V Wellsmith, G E Lindenmayer
Dec 28, 2006·Cell Biochemistry and Function·Silvana BalzanUmberto Montali
Jun 27, 2006·Journal of Pharmacological Sciences·Victor A MaltsevEdward G Lakatta
Sep 1, 1977·British Journal of Pharmacology·M BentfeldD Proppe
Jun 7, 1995·Molecular and Cellular Biochemistry·A BreierA Vrbanová

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