Abstract
This study was designed to investigate the effects of desmin oxidation on its degradation by proteolytic enzymes. Desmin was isolated from bovine muscle and exposed to varying oxidative conditions, and then incubated with μ-calpain, caspase-3 or -6, respectively. The extent of protein degradation was subsequently determined using SDS-PAGE and Western-blotting. Furthermore, the oxidative modification of the secondary structure of desmin was measured by circular dichroism (CD). Our results revealed that, compared with the native desmin, degradation of oxidised desmin was enhanced by caspases, but suppressed by μ-calpain. The CD spectra of desmin showed that the content of α-helix decreased from 76.2% to 52% while random coil increased from 8% to 22.4% after oxidation. These findings demonstrated that oxidative modifications of desmin changed their susceptibility to μ-calpain, caspase-3 and -6 as well as their secondary structure.
References
Oct 1, 1980·European Journal of Biochemistry·N Geisler, K Weber
Sep 16, 1999·Biochemical and Biophysical Research Communications·K M McGinnisK K Wang
Apr 5, 2002·Molecular Cell·Yigong Shi
Dec 13, 2002·The Journal of Biological Chemistry·Feng ChenVincent L Cryns
Oct 13, 2004·Journal of Structural Biology·Harald BärHarald Herrmann
Oct 27, 2004·Experimental Cell Research·Denise Paulin, Zhenlin Li
Jul 20, 2005·Biochimica Et Biophysica Acta·Sharon M KellyNicholas C Price
Mar 4, 2010·Journal of Agricultural and Food Chemistry·Mario Estévez, Marina Heinonen
Jun 23, 2010·Meat Science·Elisabeth Huff LonerganSteven M Lonergan
Jul 6, 2010·Free Radical Biology & Medicine·Ashley J SmuderScott K Powers
Jul 29, 2010·Meat Science·Susumu MuroyaMette Christensen
Sep 3, 2010·Journal of Agricultural and Food Chemistry·Zelong LiuJie Chen
Aug 3, 2011·Journal of Agricultural and Food Chemistry·Feng HuangMei Xue
May 1, 2001·Meat Science·L Kristensen, P P Purslow
Nov 1, 2004·Meat Science·Caroline Pascale BaronPeter Patrick Purslow
Jul 1, 2006·Meat Science·Martine MorzelElisabeth Laville
Aug 1, 2008·Animal : an International Journal of Animal Bioscience·C M Kemp, T Parr
May 2, 2012·Meat Science·Caroline M Kemp, Tim Parr
Sep 1, 2011·Food Chemistry·Ming HuangGuanghong Zhou
Citations
Feb 24, 2016·Journal of Proteomics·Alessandro Lana, Lello Zolla
Sep 30, 2018·Antioxidants & Redox Signaling·Voahanginirina RandriamboavonjyIngrid Fleming
Jan 31, 2020·Journal of Food Science·Junwei QinJian Zhang
May 4, 2017·Journal of Animal Science·K B CarlsonS M Lonergan
Apr 29, 2020·Biomolecules·Anastasiia I Petushkova, Andrey A Zamyatnin
Jul 21, 2017·Journal of Animal Science·K B CarlsonS M Lonergan
May 25, 2020·Meat Science·Xiao LuXin Luo
Feb 6, 2019·Food Research International·Muhan ZhangWeimin Xu
Jul 28, 2020·Redox Biology·Álvaro Viedma-PoyatosDolores Pérez-Sala
Mar 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Tong XingXinglian Xu
Aug 2, 2019·Experimental Cell Research·Florence DelortSabrina Batonnet-Pichon
May 28, 2021·Food Chemistry·Pingping LiuJian Zhang