Influence of Oxygen and Glucose on Primary Metabolism and Astaxanthin Production by Phaffia rhodozyma in Batch and Fed-Batch Cultures: Kinetic and Stoichiometric Analysis.

Applied and Environmental Microbiology
Y YamaneN Nishio

Abstract

The influence of the oxygen and glucose supply on primary metabolism (fermentation, respiration, and anabolism) and astaxanthin production in the yeast Phaffia rhodozyma was investigated. When P. rhodozyma grew under fermentative conditions with limited oxygen or high concentrations of glucose, the astaxanthin production rate decreased remarkably. On the other hand, when the yeast grew under aerobic conditions, the astaxanthin production rate increased with increasing oxygen uptake. A kinetic analysis showed that the respiration rate correlated positively with the astaxanthin production rate, whereas there was a negative correlation with the ethanol production rate. The influence of glucose concentration at a fixed nitrogen concentration with a high level of oxygen was then investigated. The results showed that astaxanthin production was enhanced by an initial high carbon/nitrogen ratio (C/N ratio) present in the medium, but cell growth was inhibited by a high glucose concentration. A stoichiometric analysis suggested that astaxanthin production was enhanced by decreasing the amount of NADPH required for anabolism, which could be achieved by the repression of protein biosynthesis with a high C/N ratio. Based on these results, w...Continue Reading

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Citations

Apr 30, 2017·Bioprocess and Biosystems Engineering·Xueshan PanXueping Ling
Oct 8, 2009·Applied Microbiology and Biotechnology·Cipriano Chávez-CabreraLuis Bernardo Flores-Cotera
Oct 23, 2020·Environmental Science and Pollution Research International·Kiyotaka Y HaraKeiji Wakabayashi
Mar 29, 2014·Archives of Microbiology·Alejandra Barbachano-TorresTeresa Ponce-Noyola
Aug 4, 2004·Applied Microbiology and Biotechnology·A R Domíguez-Bocanegra, J A Torres-Muñoz
Jul 1, 2015·FEMS Yeast Research·Cipriano Chávez-CabreraLuis Bernardo Flores-Cotera
Mar 17, 2010·Journal of Industrial Microbiology & Biotechnology·Lili MiaoYing Li
Sep 16, 2008·Journal of Basic Microbiology·Marcelo BaezaVíctor Cifuentes
Jul 31, 2003·Biotechnology and Bioengineering·Christopher CannizzaroUrs von Stockar
Nov 5, 2008·Journal of Industrial Microbiology & Biotechnology·Ginka I Frengova, Dora M Beshkova
Mar 3, 2007·Applied Microbiology and Biotechnology·A R Domínguez-BocanegraJ A Torres-Muñoz
Nov 11, 2015·Microbial Cell Factories·Isabel Rodríguez Amado, José Antonio Vázquez
Nov 4, 2010·Applied Microbiology and Biotechnology·Isabell SchmidtJens Schrader
Jul 4, 2021·Applied Biochemistry and Biotechnology·Zhu Chen, Caixia Wan
Jul 25, 2021·Journal of Industrial Microbiology & Biotechnology·Luis B Flores-CoteraOscar Ulises García-Flores
Aug 27, 2021·Critical Reviews in Food Science and Nutrition·Cassamo U MussagyAdalberto Pessoa
Sep 8, 2021·Microbial Cell Factories·Alejandro Torres-HaroMelchor Arellano-Plaza

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