Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

International Journal of Molecular Sciences
Piergiorgio GentileZebunnissa Ramtoola

Abstract

Biodegradable poly(lactide-co-glycolide) (PLGA) nanoparticles, containing human parathyroid hormone (PTH (1-34)), prepared by a modified double emulsion-solvent diffusion-evaporation method, were incorporated in porous freeze-dried chitosan-gelatin (CH-G) scaffolds. The PTH-loaded nanoparticles (NPTH) were characterised in terms of morphology, size, protein loading, release kinetics and in vitro assessment of biological activity of released PTH and cytocompatibility studies against clonal human osteoblast (hFOB) cells. Structural integrity of incorporated and released PTH from nanoparticles was found to be intact by using Tris-tricine SDS-PAGE. In vitro PTH release kinetics from PLGA nanoparticles were characterised by a burst release followed by a slow release phase for 3-4 weeks. The released PTH was biologically active as evidenced by the stimulated release of cyclic AMP from hFOB cells as well as increased mineralisation studies. in vitro and cell studies demonstrated that the PTH bioactivity was maintained during the fabrication of PLGA nanoparticles and upon release. Finally, a content of 33.3% w/w NPTHs was incorporated in CH-G scaffolds, showing an intermittent release during the first 10 days and, followed by a control...Continue Reading

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Citations

Apr 20, 2016·International Journal of Molecular Sciences·Bo YinZhihong Wu
Nov 30, 2016·International Journal of Molecular Sciences·Ting YuJian Yang
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Feb 14, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jae-Woo ChoJong-Keon Oh

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Methods Mentioned

BETA
electrophoresis
Assay

Software Mentioned

it
SkanIt
Analyse
Varioskan Flash

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