Influence of parenteral iron preparations on non-transferrin bound iron uptake, the iron regulatory protein and the expression of ferritin and the divalent metal transporter DMT-1 in HepG2 human hepatoma cells

Biochemical Pharmacology
Barbara Scheiber-MojdehkarHans Goldenberg

Abstract

It is widely assumed that standard parenteral iron preparations are degraded in the reticuloendothelial cells and that the iron is subsequently incorporated into transferrin. Hepatocytes or other epithelial cells have been considered as not affected. We show that this picture should be carefully reconsidered. By using the human hepatoma cell line HepG2 we showed that the parenteral iron preparations ferric saccharate and ferric gluconate donated iron to the cells as efficiently as low molecular weight iron and stimulated non-transferrin bound iron uptake. This led to inactivation of the iron regulatory protein 1 and to an increase in the expression of ferritin and of the divalent metal transporter (DMT-1). Ferric dextran was only a weak stimulator of ferritin and DMT-1 expression. The observed changes in iron metabolism occurred at concentrations of parenteral iron that can also be found in the plasma of patients after i.v. infusion. We conclude that parenteral iron also influences the iron metabolism of non-reticuloendothelial cells like HepG2 cells. Further the increase in the expression of the transporter DMT-1 in HepG2 cells after iron treatment is in contrast to the regulation in the duodenum and may be involved in the upr...Continue Reading

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Citations

Sep 3, 2003·European Journal of Biochemistry·Brigitte SturmBarbara Scheiber-Mojdehkar
Feb 10, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·David Van WyckKevin Johnson
Apr 20, 2005·Journal of Gastroenterology and Hepatology·Masaki TakeoYukihiko Adachi
Feb 9, 2005·Kidney International·Brigitte SturmBarbara Scheiber-Mojdehkar
Jul 12, 2011·International Urology and Nephrology·Peter Van BurenXin J Zhou
Jun 2, 2009·Cellular and Molecular Life Sciences : CMLS·Gregory Jon Anderson, Christopher D Vulpe
Dec 7, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Susanne RothHarald Enzmann
Nov 14, 2013·Pharmaceutical Development and Technology·Monika PraschbergerBrigitte Sturm
Jul 16, 2015·Critical Reviews in Food Science and Nutrition·Marija KnezJames C R Stangoulis
May 10, 2005·Biochimica Et Biophysica Acta·D T WardD Riccardi
Oct 19, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Monika PraschbergerBarbara Scheiber-Mojdehkar
Aug 18, 2012·The Journal of Biological Chemistry·Chia-Yu WangMitchell D Knutson

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