Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis

Human Reproduction
R CosínA Estellés

Abstract

Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent benign gynaecological diseases. It has been suggested that both endometrial and peritoneal factors, related to angiogenesis and proteolysis, can be implicated in this disease. The aim of this study was to evaluate the influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with and without endometriosis. Endometrial cells were isolated, cultured and treated with endometriotic or normal peritoneal fluid. Vascular endothelial growth factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels were evaluated by quantitative RT-PCR, and protein levels were quantified by ELISA. Peritoneal fluid from women with endometriosis induced an increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in endometrial cell culture from women with (P < 0.01) and without endometriosis (P < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell cultures from patients with endometri...Continue Reading

References

Aug 1, 1996·Seminars in Reproductive Endocrinology·K G OsteenK L Sharpe-Timms
Aug 4, 1998·Human Reproduction·J DonnezM Nisolle
Feb 23, 1999·Human Reproduction Update·P R KoninckxD H Barlow
Feb 29, 2000·Molecular Human Reproduction·D I LebovicR N Taylor
Mar 11, 2000·Human Reproduction Update·J McLaren
Apr 13, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M SillemB Runnebaum
Jul 14, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M S Pepper
Oct 12, 2001·Annals of the New York Academy of Sciences·K L Sharpe-Timms
Apr 13, 2002·Annals of the New York Academy of Sciences·Robert N TaylorMichael D Mueller
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Nov 16, 2004·Lancet·Linda C Giudice, Lee C Kao
Jan 26, 2005·Human Pathology·Mikio TakeharaMinoru Ueki
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Juan Gilabert-EstellesAmparo Estelles
Apr 12, 2005·Fertility and Sterility·Yasushi KawanoIsao Miyakawa
Oct 8, 2005·Angiogenesis·Jane E Girling, Peter A W Rogers
Mar 10, 2007·Human Reproduction Update·Matthias W Laschke, Michael D Menger
May 14, 2008·Current Medicinal Chemistry·Esther ZorioAmparo Estellés

❮ Previous
Next ❯

Citations

Feb 22, 2011·Human Reproduction·Luis A RamónAmparo Estellés
Mar 13, 2014·Human Reproduction·Aitana Braza-BoïlsJuan Gilabert-Estellés
Jun 15, 2013·Obstetrics and Gynecology International·Ana Luiza L RochaRobert N Taylor
Apr 24, 2015·Thrombosis Research·Josep Marí-AlexandreJuan Gilabert-Estellés
Jun 1, 2016·BioMed Research International·Ningning WangBin Liu
Nov 5, 2011·Reproductive Sciences·Liqing ChenXinmei Zhang
Mar 12, 2016·Clinical Medicine Insights. Women's Health·Cristina M De la Hera-LazaroJesús S Jiménez-López
Aug 15, 2018·International Journal of Molecular Sciences·Yi-Heng LinYen-Hua Huang
Apr 2, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Haiyuan Liu, Jing He Lang
Nov 17, 2019·International Journal of Molecular Sciences·Josep Marí-AlexandreJuan Gilabert-Estellés
Oct 29, 2020·Reproductive Biomedicine Online·Tugba Ekiz-YilmazElif Guzel
Jun 3, 2021·International Journal of Molecular Sciences·Irene JiangMohamed A Bedaiwy
Jul 28, 2020·Journal of Reproductive Immunology·Navid ShomaliMehdi Yousefi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.