Dec 1, 2005

Influence of pH, buffer species, and storage temperature on physicochemical stability of a humanized monoclonal antibody LA298

International Journal of Pharmaceutics
Jack Y Zheng, Linda J Janis

Abstract

The purpose of this work is to study the effect of pH, buffer species, and temperature on the physicochemical stability of a humanized monoclonal antibody LA298. The study was carried out in solution state of the antibody in the presence of different buffer species at different pH values and storage temperature. No significant changes in total protein content were observed for any of the solutions with different buffers at different pH values when stored for 8 weeks at both 5 degrees C and 25 degrees C or at 37 degrees C for 1 week. Known asparagines (Asn55) deamidation of LA298 was found to be dependent on pH, buffer type, and temperature. The estimated rate constant of the double heavy chain Asn55 deamidation in phosphate buffer at pH 6.5 and 7.0 was much higher than that in citrate buffer under the same storage conditions. However, comparable results were obtained for single heavy chain Asn55 deamidation in citrate and phosphate buffer. Aggregation of LA298 was not significant for samples at different pH values, buffers, and temperatures as the monomer of LA298 decreased dramatically over time. Less decrease in monomeric LA298 was observed in citrate buffer, pH 5.0-5.5. In conclusion, to minimize deamidation and loss of LA29...Continue Reading

Mentioned in this Paper

Buffers
Monoclonal Antibodies
Chinese Hamster
Asparagine
Biochemical Pathway
Covalent Interaction
IgG1 Antibody
Phosphate buffers
Citrate buffer
Aggregation

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