Influence of pH on Ammonia Accumulation and Toxicity in Halophilic, Methylotrophic Methanogens.

Applied and Environmental Microbiology
P C Kadam, D R Boone

Abstract

We studied the effects of pH and ammonia concentration on the growth of three methanogens. These three halophilic, methylotrophic methanogens, Methanolobus bombayensis, Methanolobus taylorii, and Methanohalophilus zhilinaeae, grew at environmental pH ranges that overlapped with each other and spanned the pH range from 7.0 to 9.5. During growth they had reversed membrane pH gradients ((Delta)pH) at all pH values tested. The (Delta)pH was in the range of -0.4 to -0.9 pH units, with the cytosol being more acidic than the environmental pH. Methanohalophilus zhilinaeae had the most negative (Delta)pH (-0.9 pH units). These negative pH gradients resulted in the accumulation of ammonium (NH(inf4)(sup+)), and when grown at the highest external ammonia concentrations that allowed good growth, cells had cytosolic NH(inf4)(sup+) concentrations as high as 180 mM. The high concentrations of cytosolic NH(inf4)(sup+) were accompanied by greater (Delta)pH and lower concentrations of the major cytosolic cation K(sup+) (compared with cells grown in medium with only 5 mM ammonia). Methanolobus bombayensis and Methanolobus taylorii were more sensitive to total external ammonia at higher external pH values, but the inhibitory concentration of un-io...Continue Reading

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Citations

Apr 12, 2006·Applied Microbiology and Biotechnology·Manoj K TiwariShweta Tripathi
Feb 16, 2010·Applied and Environmental Microbiology·E NettmannM Klocke
Dec 25, 2009·FEMS Microbiology Ecology·Philipp LinsPaul Illmer
Jan 1, 2015·Microbiology Insights·Kaushik VenkiteshwaranDaniel Zitomer
Jun 5, 2016·Applied and Environmental Microbiology·Loïc DecreyTamar Kohn
Dec 16, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Li YunWang Xiujie
Aug 18, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Seyong ParkMoonil Kim
Dec 20, 2013·Environmental Technology·Anupama Vijaya NadarajaKrishnakumar Bhaskaran
Aug 28, 2020·Environmental Science & Technology·Miao YanIrini Angelidaki
Dec 12, 2017·FEMS Microbiology Letters·Pablo LedezmaStefano Freguia
Sep 27, 2019·Frontiers in Microbiology·Martin A FischerRuth A Schmitz
Nov 25, 2020·The Science of the Total Environment·Farrukh Raza AminRuihong Zhang
Oct 20, 2018·Applied Microbiology and Biotechnology·Zuopeng LvMarcell Nikolausz
Jun 13, 2002·Bioresource Technology·E Salminen, J Rintala

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