Influence of physical factors on the growth of insect cells in vitro. II. Sodium and potassium as osmotic pressure regulators of moth cell growth.

In Vitro
T J KurttiM A Brooks

Abstract

The tolerance of a cell line (IMC-HZ-1) from a moth, Heliothis zea, for the monovalent cations Na+ and K+ were defined. Cells shifted to media containing more than 70 mM of K+ showed decreased growth rates. No evidence was obtained for Na+ toxicity. The osmotic pressure tolerances were influenced by the K+ concentration of the medium. The richer the medium was in K+, the narrower was the spectrum of osmotic pressure tolerance. Once the limit of K+ tolerance was exceeded, the rate of decline of growth was linear with respect to further increases in K+. This rate of decline was independent of osmotic pressure. The initial responses of cells during one subculture (2 to 4 population doublings) in media differing from the standard medium (used to maintain the cell line) were not reliable indicators of the growth potential of the cells. Continued subculture in such media resulted in an upward trend in population growth rates in most cases.

References

Jan 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C W OrrJ D Ebert
Oct 1, 1970·Physiological Reviews·S G Schultz, P F Curran
Jun 1, 1972·Journal of Insect Physiology·E Cohen, L I Gilbert
Feb 1, 1972·Experimental Cell Research·D O Schachtschabel, G E Foley
Apr 1, 1956·Archives of Biochemistry and Biophysics·H EAGLE
Aug 1, 1963·Biochemical and Biophysical Research Communications·H M NITOWSKYS GELLER
Feb 1, 1964·Experimental Cell Research·S J PIRT, E J THACKERAY

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Citations

Jun 1, 1997·In Vitro Cellular & Developmental Biology. Animal·G L HolbrookC Schal
Jan 12, 2010·In Vitro Cellular & Developmental Biology. Animal·Yoshitake AkiyamaKeisuke Morishima
Jan 1, 1996·Cytotechnology·G Schmid
Sep 5, 2012·Biomedical Microdevices·Yoshitake AkiyamaKeisuke Morishima
May 1, 1982·Biotechnology and Bioengineering·S A WeissE M Dougherty

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