Influence of physiologic folate deficiency on human papillomavirus type 16 (HPV16)-harboring human keratinocytes in vitro and in vivo.

The Journal of Biological Chemistry
Suhong XiaoAśok C Antony

Abstract

Although HPV16 transforms infected epithelial tissues to cancer in the presence of several co-factors, there is insufficient molecular evidence that poor nutrition has any such role. Because physiological folate deficiency led to the intracellular homocysteinylation of heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1) and activated a nutrition-sensitive (homocysteine-responsive) posttranscriptional RNA operon that included interaction with HPV16 L2 mRNA, we investigated the functional consequences of folate deficiency on HPV16 in immortalized HPV16-harboring human (BC-1-Ep/SL) keratinocytes and HPV16-organotypic rafts. Although homocysteinylated hnRNP-E1 interacted with HPV16 L2 mRNA cis-element, it also specifically bound another HPV16 57-nucleotide poly(U)-rich cis-element in the early polyadenylation element (upstream of L2L1 genes) with greater affinity. Together, these interactions led to a profound reduction of both L1 and L2 mRNA and proteins without effects on HPV16 E6 and E7 in vitro, and in cultured keratinocyte monolayers and HPV16-low folate-organotypic rafts developed in physiological low folate medium. In addition, HPV16-low folate-organotypic rafts contained fewer HPV16 viral particles, a similar HPV16 DNA vi...Continue Reading

References

Feb 1, 1992·JAMA : the Journal of the American Medical Association·C E ButterworthV V Baker
Dec 7, 1984·Science·J J Yunis, A L Soreng
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M GoulianB Y Tseng
Jan 1, 1982·The American Journal of Clinical Nutrition·C E ButterworthC L Krumdieck
Mar 1, 1980·Archives of Biochemistry and Biophysics·G B Henderson, E M Zevely
Jun 1, 1995·Current Opinion in Genetics & Development·G R Sutherland, R I Richards
Aug 1, 1993·Journal of the American College of Nutrition·C E Butterworth
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Jeon, P F Lambert
Jan 1, 1997·The American Journal of Clinical Nutrition·Y I KimJ B Mason
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·B C BlountB N Ames
Aug 1, 1997·Journal of Virology·L B BlynE Ehrenfeld
Sep 1, 1997·Molecular and Cellular Biology·B StefanovicD A Brenner
Apr 29, 1998·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·M KalantariB Johansson
Dec 16, 1998·Trends in Biochemical Sciences·A Ostareck-LedererM W Hentze
Jun 14, 2000·European Journal of Pharmacology·S B PhagooG M Burgess
Jul 6, 2000·Lancet·C Johnston
Jan 6, 2001·Nutrition and Cancer·S W ThomsonC E Butterworth
Jul 18, 2001·Cancer Causes & Control : CCC·S J WeinsteinP D Stolley
Jul 27, 2001·The American Journal of Clinical Nutrition·A C Antony
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Sep 5, 2002·Nature Reviews. Cancer·Bruce N Ames, Patricia Wakimoto
Jun 21, 2003·The American Journal of Clinical Nutrition·Aśok C Antony
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Michelle S Longworth, Laimonis A Laimins
Jun 11, 2004·Annual Review of Nutrition·Sally P Stabler, Robert H Allen

❮ Previous
Next ❯

Citations

Nov 15, 2018·Physiological Reviews·Hieronim Jakubowski
Jul 23, 2015·EMBO Molecular Medicine·Noa LammBatsheva Kerem

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.