Influence of plasma protein on the inhibitory effects of indocyanine green and bromcresol green on pulmonary prostaglandin E1 extraction

British Journal of Pharmacology
C A DawsonD L Roerig

Abstract

The purpose of this study was to examine the influence of plasma protein on the inhibitory effects of the anionic dyes indocyanine green and bromcresol green on prostaglandin E1 (PGE1) uptake by the lungs. Dog lung lobes were isolated and perfused with either autologous plasma or Krebs-Ringer bicarbonate solution (KRB) containing no protein but with dextran used as a colloid. PGE1 uptake was determined by injecting a bolus, containing radiolabelled PGE1 into the lobar artery and then analysing ethanolic extracts of the venous effluent for radioactivity in PGE1 and PGE1 metabolites by thin layer chromatography and scintillation counting. When the lobes were perfused with KRB, bromcresol green at an average initial concentration of 28.5 microM, reduced PGE1 by an average of 56%. When the lobes were perfused with plasma, similar concentrations of bromcresol green reduced the uptake by less than 2%. A similar result was obtained with indocyanine green, which at an average initial concentration of 17.5 microM reduced uptake by about 70% when the lobes were perfused with KRB, but when the lobes were perfused with plasma similar concentrations of the dye reduced uptake by less than 3.5%. The results suggest that plasma protein binding...Continue Reading

References

Apr 1, 1977·Prostaglandins·B O Cozzini, C A Dawson
Aug 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J H Linehan, C A Dawson
Feb 1, 1978·British Journal of Pharmacology·Y S BakhleB J Whittle
Apr 1, 1976·Journal of Applied Physiology·M L LandsmanW G Zijlstra
Apr 1, 1977·Canadian Journal of Physiology and Pharmacology·B O Cozzini, C A Dawson
Aug 1, 1975·Canadian Journal of Physiology and Pharmacology·C A DawsonA J Lonigro
Jun 19, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·K KamisakaH Kameda
Dec 18, 1974·Biochimica Et Biophysica Acta·G DeutschmannV Ullrich
Sep 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·M R TrippI J Fox
Aug 17, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·H KawasakiC Hirayama
Aug 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·K J Baker
Jun 1, 1982·British Journal of Pharmacology·P G Hellewell, J D Pearson
Nov 1, 1983·Prostaglandins, Leukotrienes, and Medicine·R A Forder, F Carey
Jan 1, 1982·Annals of the New York Academy of Sciences·F R LewisJ M Christensen
Apr 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·B R PittC N Gillis
Feb 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J H LinehanV M Wagner-Weber
Aug 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·D A RickabyC A Dawson
Oct 1, 1956·Archives of Biochemistry and Biophysics·K SCHILLING, H WALDMANN-MEYER
Apr 1, 1960·The Journal of Clinical Investigation·G R CHERRICKC S DAVIDSON

❮ Previous
Next ❯

Citations

Apr 29, 1998·Annual Review of Physiology·V L Schuster
Apr 1, 2009·Acta Veterinaria Scandinavica·Xiaofeng LiJinping Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved