Influence of plasmid concentration on DNA electrotransfer in vitro using high-voltage and low-voltage pulses.

The Journal of Membrane Biology
Karolina CepurnieneSaulius Satkauskas

Abstract

DNA electrotransfer in vivo for gene therapy is a promising method. For further clinical developments, the efficiency of the method should be increased. It has been shown previously that high efficiency of gene electrotransfer in vivo can be achieved using high-voltage (HV) and low-voltage (LV) pulses. In this study we evaluated whether HV and LV pulses could be optimized in vitro for efficient DNA electrotransfer. Experiments were performed using Chinese hamster ovary (CHO) cells. To evaluate the efficiency of DNA electrotransfer, two different plasmids coding for GFP and luciferase were used. For DNA electrotransfer experiments 50 microl of CHO cell suspension containing 100, 10 or 1 microg/ml of the plasmid were placed between plate electrodes and subjected to various combinations of HV and LV pulses. The results showed that at 100 microg/ml plasmid concentration LV pulse delivered after HV pulse increased neither the percentage of transfected cells nor the total transfection efficiency (luciferase activity). The contribution of the LV pulse was evident only at reduced concentration (10 and 1 microg/ml) of the plasmid. In comparison to HV (1,200 V/cm, 100 micros) pulse, addition of LV (100 V/cm, 100 ms) pulse increased trans...Continue Reading

References

Oct 1, 1991·Biophysical Journal·V A Klenchin Chizmadzhev YuA
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Muriel GolzioMarie-Pierre Rols
Feb 7, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Saulius SatkauskasLluis M Mir
Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
Nov 1, 2006·Current Pharmaceutical Design·M CemazarJ Teissié
Sep 8, 2007·Journal of Pharmaceutical Sciences·Shigeru KawakamiMitsuru Hashida
Sep 9, 2008·British Journal of Pharmacology·D BouardF-L Cosset
Mar 12, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yiqi Seow, Matthew J Wood
Jul 10, 2009·The New England Journal of Medicine·Karen M FrankJohn Hart

❮ Previous
Next ❯

Citations

Jul 28, 2012·The Journal of Membrane Biology·J OrioJ Teissie
Apr 1, 2014·Biomedical Microdevices·Tomás García-SánchezRamon Bragós
Feb 22, 2012·Expert Opinion on Biological Therapy·Saulius SatkauskasMindaugas S Venslauskas
Aug 20, 2014·The Journal of Membrane Biology·L PasquetJ Teissie
Jun 17, 2015·The Journal of Membrane Biology·Baltramiejus JakštysSaulius Šatkauskas
Mar 13, 2015·The Journal of Gene Medicine·Sandra SungailaitėSaulius Šatkauskas
Mar 2, 2012·Technology in Cancer Research & Treatment·M TamosiūnasS Satkauskas
Oct 21, 2018·Scientific Reports·Paulius RuzgysSaulius Šatkauskas
Nov 3, 2020·Frontiers in Genetics·Luiz Sergio Almeida CamargoPablo Juan Ross

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Membrane Biology
Mojca PavlinMasa Kanduser
Journal of Controlled Release : Official Journal of the Controlled Release Society
N Pavselj, Véronique Préat
Molecular Therapy : the Journal of the American Society of Gene Therapy
Saulius SatkauskasLluis M Mir
© 2021 Meta ULC. All rights reserved