Influence of Poly(L-Lactic Acid) Aligned Nanofibers on PC12 Differentiation

Journal of Biomedical Nanotechnology
Yadong YuFei Ding

Abstract

The aim of this study was to unveil the mechanism by which aligned nanofibers influence neuronal differentiation. PC12 cells were seeded on three different poly(L-lactic acid) (PLLA) substrates (PLLA films (control), electrospun PLLA random nanofibers (RF) and electrospun PLLA aligned nanofibers (AF)). Subsequently, cellular experiments, cDNA microarrays and molecular biological approaches were employed to investigate the impacts of the different PLLA substrates on PC12 cell differentiation. Scanning electron microscope observation revealed that neurite outgrowth in the AF group was parallel to the direction of nanofiber alignment and that the filopodias at the neurite tips spread along the aligned nanofiber axis. Meanwhile, both neurite length and the expression of GAP43 (a neuronal differentiation marker gene) were higher in the AF group than those in the control and RF groups. These results suggested that the PLLA aligned nanofibers enhanced PC12 cell differentiation. cDNA microarray experiment revealed that 876 and 1937 genes had significantly changed expression in the RF and AF groups, respectively. Based on gene ontology analysis, 493 and 1193 differentially expressed genes involved in neuronal differentiation were found ...Continue Reading

Citations

Feb 16, 2016·Carbohydrate Polymers·Marziyeh Ranjbar-MohammadiSeeram Ramakrishna
May 24, 2018·Nanomedicine·Chen AijieShao Longquan
Feb 11, 2018·Journal of Biomedical Materials Research. Part a·Ulku Selcen DemirKezban Ulubayram
Nov 14, 2016·ACS Biomaterials Science & Engineering·Yaopeng ZhangLujie Song

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