Influence of pressure on structure and dynamics of bovine pancreatic trypsin inhibitor (BPTI): small angle and quasi-elastic neutron scattering studies

Biochimica Et Biophysica Acta
M-S AppavouM-C Bellissent-Funel

Abstract

We have studied the influence of pressure on structure and dynamics of a small protein belonging to the enzymatic catalysis: the bovine pancreatic trypsin inhibitor (BPTI). Using a copper-beryllium high-pressure cell, we have performed small angle neutron scattering (SANS) experiment on NEAT spectrometer at HMI (Berlin, Germany). In the SANS configuration, the evolution of the radius of gyration and of the shape of the protein under pressures up to 6,000 bar has been studied. When increasing pressure from atmospheric pressure up to 6,000 bar, the pressure effects on the global structure of BPTI result on a reduction of the radius of gyration from 13.4 A down to 12.0 A. Between 5,000 and 6,000 bar, some transition already detected by FTIR [N. Takeda, K. Nakano, M. Kato, Y. Taniguchi, Biospectroscopy, 4, 1998, pp. 209-216] is observed. The pressure effect is not reversible because the initial value of the radius of gyration is not recovered after pressure release. By extending the range of wave-vectors to high q, we have observed a change of the form factor (shape) of the BPTI under pressure. At atmospheric pressure BPTI exhibits an ellipsoidal form factor that is characteristic of the native state. When the pressure is increased...Continue Reading

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Citations

Jul 10, 2009·Journal of the Royal Society, Interface·Marion Jasnin
Jan 17, 2012·Biochimica Et Biophysica Acta·Koji YoshidaMarie-Claire Bellissent-Funel
Jan 10, 2009·Chemical Physics·S C M TeixeiraG Zaccai
May 28, 2014·Biochimica Et Biophysica Acta·Gabriel GibratMarie-Claire Bellissent-Funel
Jun 23, 2009·Biochimica Et Biophysica Acta·G R Kneller, V Calandrini
Nov 29, 2013·Chemical Communications : Chem Comm·Filip Meersman, Paul F McMillan
Jun 4, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S ChodankarA G Wagh
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daniela RussoMatthias Heyden
Jul 16, 2019·Biochimica Et Biophysica Acta. General Subjects·Hiroaki HataAkio Kitao
Feb 23, 2011·The Journal of Physical Chemistry. B·Ronald GebhardtWolfgang Doster

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