PMID: 2489053Feb 1, 1989Paper

Influence of protein conformation on its adaptability under chaotropic conditions

International Journal of Biological Macromolecules
S Damodaran

Abstract

The thermostability of serum albumin and beta-lactoglobulin in various salt solutions was studied using differential scanning calorimetry. Below 1.0 M salt concentrations, the relative effectiveness of various sodium salts on increasing the thermostability of beta-lactoglobulin followed the classic Hofmeister or lyotropic series, i.e. SO2-(4) greater than Cl- greater than Br- greater than ClO-4 greater than SCN-; however, in the case of serum albumin the above order was reversed, i.e. ClO-4 greater than SCN- greater than Br- greater than Cl- greater than SO2-(4), indicating that the thermostability of serum albumin was higher in chaotropic solution conditions. Circular dichroic analysis of serum albumin in NaClO4 solutions revealed that the alpha-helical content of the protein increased from 59% to 73% in 1.0 M NaClO4; no similar increase in secondary structure was observed for beta-lactoglobulin. These observations contradicted the general notion that the chaotropic effect of neutral salts on the stability of macromolecules is independent of any details of the macromolecular conformation itself. The results presented here indicate that the predisposition of the native conformation of a protein per se might affect whether the p...Continue Reading

References

Jun 25, 1979·Journal of Molecular Biology·D W GreenK F Woods
Nov 1, 1977·Physics in Medicine and Biology·C G EssexH Peeters
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·Y Hatefi, W G Hanstein
Apr 1, 1970·Archives of Biochemistry and Biophysics·H B Bull, K Breese
Mar 27, 1968·Journal of the American Chemical Society·H Inoue, S N Timasheff
Nov 1, 1983·Archives of Biochemistry and Biophysics·L K CreamerG N Malcolm
Jul 1, 1983·Archives of Biochemistry and Biophysics·T Arakawa, S N Timasheff

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Citations

Mar 20, 1999·Biochimica Et Biophysica Acta·J W TannerP A Liebman
Oct 23, 2009·Biochemistry·Kan XiongSanford A Asher
May 5, 2009·International Journal of Biological Macromolecules·D BarrecaMark T F Telling
Sep 9, 2009·Colloids and Surfaces. B, Biointerfaces·Janet R WendorfHarvey W Blanch
Nov 1, 1996·Biopolymers·M A Lillie, J M Gosline
Dec 1, 1991·International Journal of Biological Macromolecules·M YamasakiK Aoki
May 15, 1990·Archives of Biochemistry and Biophysics·R A Wolosiuk, M Stein
Oct 26, 2013·Biochemistry·Srinivasan Damodaran
Sep 13, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Lei Zhao, Srinivasan Damodaran
Jun 1, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Srinivasan Damodaran

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