PMID: 6685223Jan 1, 1983

Influence of protein net charge on the nucleic acid helix-destabilizing activity of various pancreatic ribonucleases

Molecular and Cellular Biochemistry
A CarsanaM Libonati


Helix-destabilization of double-stranded poly[d(A-T)]induced by various homologous pancreatic ribonucleases which differ in their net charges has been studied under different ionic strength conditions. The response of the destabilizing activity of the various proteins to ionic strength is represented by bell-shaped curves, whose maxima are shifted to higher ionic strength values the higher the number of positive charges of the RNAase involved in the nucleic acid-protein complex. This observation is discussed, and a model proposed, that could explain the experimental results presented.


Sep 22, 2001·Protein Science : a Publication of the Protein Society·A NenciM Libonati
Aug 1, 2016·Journal of Photochemistry and Photobiology. B, Biology·Amit Kumar DindaSwagata Dasgupta

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