Influence of quercetin on the interaction of gliclazide with human serum albumin - spectroscopic and docking approaches

Luminescence : the Journal of Biological and Chemical Luminescence
Miroslava KameníkováMilan Nagy

Abstract

Protein-binding interactions are displacement reactions which have been implicated as the causative mechanisms in many drug-drug interactions. Thus, the aim of presented study was to analyse human serum albumin-binding displacement interaction between two ligands, hypoglycaemic drug gliclazide and widely distributed plant flavonoid quercetin. Fluorescence analysis was used in order to investigate the effect of substances on intrinsic fluorescence of human serum albumin (HSA) and to define binding and quenching properties of ligand-albumin complexes in binary and ternary systems, respectively. Both ligands showed the ability to bind to HSA, although to a different extent. The displacement effect of one ligand from HSA by the other one has been described on the basis of the quenching curves and binding constants comparison for the binary and ternary systems. According to the fluorescence data analysis, gliclazide presents a substance with a lower binding capacity towards HSA compared with quercetin. Results also showed that the presence of quercetin hindered the interaction between HSA and gliclazide, as the binding constant for gliclazide in the ternary system was remarkably lower compared with the binary system. This finding in...Continue Reading

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Citations

Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zuzana VanekováMilan Nagy
Feb 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zuzana VanekováMilan Nagy
Jul 10, 2018·Journal of Agricultural and Food Chemistry·Danielle R GonçalvesThais B Cesar
Nov 11, 2020·Current Protein & Peptide Science·Peiyu XueLi Ren

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