Influence of recipient cytoplasm cell stage on transcription in bovine nucleus transfer embryos

Molecular Reproduction and Development
S D SmithH Callesen

Abstract

Nucleus transfer for the production of multiple embryos derived from a donor embryo relies upon the reprogramming of the donor nucleus so that it behaves similar to a zygotic nucleus. One indication of nucleus reprogramming is the RNA synthetic activity. In normal bovine embryogenesis, the embryo relies upon maternally derived RNA transcripts up to the 8-cell stage, at which time it begins to transcribe its own RNA. In this experiment, RNA synthesis was detected in nucleus transfer embryos (NTE) and control embryos by pulsing with 3H-uridine, fixation, and autoradiography on semithin sections. NTE were produced using either a MII phase (nonactivated) cytoplasts at 32 hr of maturation or S-phase (activated) cytoplasts activated with calcium ionophore A23187 and cycloheximide treatment approximately 8 hr prior to fusion with a blastomere from an in-vitro-produced morula stage embryo at 32 hr of maturation. Control in-vitro-produced embryos were 3H-uridine-labelled and fixed at the 2-, 4-, early 8-, and late 8-cell stages. NTE were similarly prepared at 1, 3, and 20 hr postfusion and at the 2-, 4-, and 8-cell stages. In the control embryos, RNA synthesis was absent in the 2-, 4-, and early 8-cell stages, whereas in all late 8-cell...Continue Reading

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Citations

Aug 26, 1998·Molecular Reproduction and Development·P A De SousaS Bilodeau-Goeseels
Apr 17, 1999·Molecular Reproduction and Development·J KankaH Callesen
Aug 24, 2000·Molecular Reproduction and Development·R AlberioV Zakhartchenko
Oct 13, 2001·Molecular Reproduction and Development·R DanielsA O Trounson
Apr 21, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Valeria MericoSilvia Garagna
Oct 13, 2005·Cloning·P A De SousaM E Westhusin
Aug 16, 2002·Cloning and Stem Cells·Megan MunsiePeter Mountford
May 23, 2002·Genes & Development·Michele BoianiK John McLaughlin
Sep 26, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Andrey PichuginNathalie Beaujean
Mar 14, 2002·Molecular Reproduction and Development·J LaurincikP Maddox-Hyttel
Sep 18, 2012·Theriogenology·N Rodriguez-OsorioE Memili
May 1, 1998·Zygote : the Biology of Gametes and Early Embryos·J M CumminsR Yanagimachi
Jan 24, 2002·Biology of Reproduction·V BaranJ E Fléchon
Mar 29, 2014·Journal of Veterinary Science·Jongki ChoByeong Chun Lee
Apr 15, 2008·Zygote : the Biology of Gametes and Early Embryos·Yong TaoPoul Maddox-Hyttel
Apr 12, 1997·The Veterinary Record·P J BoothH Callesen

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