PMID: 2480341Nov 1, 1989Paper

Influence of retention time on degradation of pancreatic enzymes by human colonic bacteria grown in a 3-stage continuous culture system.

The Journal of Applied Bacteriology
G T MacfarlaneG R Gibson

Abstract

Hydrolytic enzymes were measured in gut contents from four sudden death victims. Pancreatic amylase and total protease activities decreased distally from the small bowel to the sigmoid/rectum region of the large intestine, showing that considerable breakdown or inactivation of the enzymes occurred during gut transit. To determine whether pancreatic enzymes were substrates for the gut microflora, mixed populations of bacteria were grown in a 3-stage continuous culture system on a medium that contained pancreatic extract as the sole nitrogen source. The multichamber system (MCS) was designed to reproduce in vitro, the low pH, high nutrient, fast growth conditions of the caecum and right colon and the neutral pH, low nutrient, slow growth conditions of the left colon. Results showed that pancreatic amylase was resistant to breakdown by intestinal bacteria compared with the peptide hydrolases in pancreatic secretions. Leucine aminopeptidase, trypsin and to a lesser degree, chymotrypsin, were easily degraded by gut bacteria, but pancreatic elastase was comparatively resistant to breakdown. Protein degradation in the MCS, as determined by enzyme activities, protein concentration and ammonia and phenol production, increased concomitan...Continue Reading

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Citations

Nov 16, 2002·Archives of Pharmacal Research·Hye J Lee
Jan 1, 1992·Critical Reviews in Food Science and Nutrition·C J Rumney, I R Rowland
Jan 1, 1992·The Journal of Applied Bacteriology·G T MacfarlaneJ H Cummings
Nov 5, 2005·Applied and Environmental Microbiology·Sandra MacfarlaneGeorge T Macfarlane
May 2, 2014·Seminars in Nephrology·Björn MeijersStephan J L Bakker
Apr 22, 2015·Nutrients·Evelien P J G NeisSander S Rensen
Jan 16, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anne-Marie DavilaDaniel Tomé
Nov 28, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anne-Marie DavilaDaniel Tomé
Aug 7, 2008·International Journal of Pharmaceutics·Tiago SousaAbdul W Basit
Dec 7, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Libo Yang
Jan 30, 2014·Environmental Microbiology·Sandra Macfarlane
Jul 1, 1997·Research in Veterinary Science·J A Lowe, S J Kershaw
Jul 6, 2004·Journal of Chromatography. a·Stephanie WilligRolf Claus
Jun 19, 2013·Best Practice & Research. Clinical Gastroenterology·Koen Venema, Pieter van den Abbeele
Jan 21, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Niranjan Goud KotlaAshwini Shivapooja
Apr 27, 2013·Pathogens and Disease·Erik C von RosenvingeMark E Shirtliff
Aug 27, 2016·Nature Microbiology·Henrik M RoagerTine R Licht
Apr 2, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sachin Kumar SinghYogyata Vaidya
Dec 1, 2009·Kidney International. Supplement·Pieter EvenepoelKristin Verbeke
Apr 1, 1997·Advances in Dental Research·S MacfarlaneG T Macfarlane
Oct 19, 2017·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·David HoughtonLaura C Greaves
Apr 24, 2007·Journal of Applied Microbiology·E J Woodmansey
Jun 1, 1991·The Journal of Applied Bacteriology·J H Cummings, G T Macfarlane
Feb 9, 2018·Frontiers in Microbiology·Kevin D'hoeJeroen Raes
Apr 25, 2018·Mediators of Inflammation·Anna PiccaEmanuele Marzetti
Sep 1, 1997·Journal of Pediatric Gastroenterology and Nutrition·C Guérin-DananO Szylit
Jul 31, 2002·Applied and Environmental Microbiology·Sylvia H DuncanColin S Stewart
Jun 20, 2015·Applied Microbiology and Biotechnology·Prem Prashant ChaudharyJean-François Brugère

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