Influence of RNA Strand Rigidity on Polyion Complex Formation with Block Catiomers

Macromolecular Rapid Communications
Kotaro HayashiKazunori Kataoka

Abstract

Polyion complexes (b-PICs) are prepared by mixing single- or double-stranded oligo RNA (aniomer) with poly(ethylene glycol)-b-poly(L-lysine) (PEG-PLL) (block catiomer) to clarify the effect of aniomer chain rigidity on association behaviors at varying concentrations. Here, a 21-mer single-stranded RNA (ssRNA) (persistence length: 1.0 nm) and a 21-mer double-stranded RNA (small interfering RNA, siRNA) (persistence length: 62 nm) are compared. Both oligo RNAs form a minimal charge-neutralized ionomer pair with a single PEG-PLL chain, termed unit b-PIC (uPIC), at low concentrations (<≈ 0.01 mg mL(-1)). Above the critical association concentration (≈ 0.01 mg mL(-1)), ssRNA b-PICs form secondary associates, PIC micelles, with sizes up to 30-70 nm, while no such multimolecular assembly is observed for siRNA b-PICs. The entropy gain associated with the formation of a segregated PIC phase in the multimolecular PIC micelles may not be large enough for rigid siRNA strands to compensate with appreciably high steric repulsion derived from PEG chains. Chain rigidity appears to be a critical parameter in polyion complex association.

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Citations

May 2, 2016·The Journal of Chemical Physics·Jiajia ZhouFriederike Schmid
Jul 16, 2016·Advances in Colloid and Interface Science·Amanda B MarcielLorraine Leon
Oct 18, 2016·Drug Discoveries & Therapeutics·Kanjiro Miyata
Sep 4, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu YiKazunori Kataoka
Feb 23, 2020·Cancer Science·Yoshihisa TokumaruYukihiro Akao
May 6, 2020·Nanoscale·Lies A L FliervoetTina Vermonden
Apr 26, 2019·Nature Communications·Sumiyo WatanabeKazunori Kataoka
Apr 12, 2019·Drug Delivery and Translational Research·Bo LouWim E Hennink
Sep 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lies A L FliervoetTina Vermonden
Dec 18, 2020·Advanced Drug Delivery Reviews·Yong Liu, Lichen Yin
Jan 9, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mitsuru NaitoKanjiro Miyata
Jan 15, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu YiKazunori Kataoka
May 4, 2021·Chemical Reviews·Ramya KumarTheresa M Reineke
Jun 24, 2021·The Journal of Physical Chemistry. B·Alexander E MarrasMatthew V Tirrell
Jun 5, 2018·Biomacromolecules·Hyun Jin KimKanjiro Miyata
Jun 30, 2018·Chemical Reviews·Horacio CabralKazunori Kataoka
Jan 10, 2018·Journal of the American Chemical Society·Jeffrey R ViereggMatthew V Tirrell
Oct 20, 2018·Nano Letters·Michael LueckheideMatthew V Tirrell
Dec 29, 2020·Journal of the American Chemical Society·Junjie Li, Kazunori Kataoka
Sep 2, 2021·Bioactive Materials·Lei WuJun Yue

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