Influence of salt type and ionic strength on self-assembly of dextran sulfate-ciprofloxacin nanoplexes

International Journal of Pharmaceutics
Marika KutscherOliver Germershaus

Abstract

We evaluated an analytical setup to identify optimal preparation conditions for nanoplex formation of small molecule drugs and polyelectrolytes using ciprofloxacin (CIP) and dextran sulfate (DS) as model compounds. The suitability of isothermal titration calorimetry (ITC) as a screening tool for rational formulation optimization was assessed. Besides ITC, static and dynamic light scattering, zeta potential measurements and scanning electron microscopy were applied to analyze the influence of different salt types and ionic strengths on CIP/DS nanoplex formation. The addition of low amounts of salt, especially 0.1M NaCl, improved the formation of CIP/DS nanoplexes. The presence of low amounts of salt led to smaller and more numerous particles of higher uniformity but had no influence on the release of CIP from nanoplexes. Furthermore, the molar range, within which efficient complexation was achieved, was broader in the presence of 0.1M NaCl than in the absence of salt with overall comparable complexation efficiency. Importantly, binding affinity correlated with particle shape and morphology, potentially enabling optimization of critical quality attributes based on ITC data. Altogether, ITC along with supplemental methods is a ver...Continue Reading

References

Aug 25, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Christophe SchatzThierry Delair
Jun 15, 2007·International Journal of Pharmaceutics·Wei-Guo DaiYan-Qiu Song
Jul 17, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y TianKam C Tam
Jul 24, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hélder A SantosKyösti Kontturi
Nov 1, 2007·Expert Opinion on Drug Delivery·Philippe Ga Rogueda, Daniela Traini
Oct 14, 2008·Advanced Drug Delivery Reviews·Zonghua LiuZiyong Zhang
Nov 5, 2011·Journal of Colloid and Interface Science·Wean Sin Cheow, Kunn Hadinoto
Mar 24, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Wean Sin Cheow, Kunn Hadinoto
Apr 13, 2012·Physical Chemistry Chemical Physics : PCCP·Zhenli LuoGuangzhao Zhang
Sep 19, 2012·Advanced Materials·Laura M EnsignJustin Hanes
Oct 23, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Dimitrios PriftisMatthew Tirrell
Jul 17, 2013·The Journal of Physical Chemistry. B·Yan Huang, Yakov Lapitsky
Jul 23, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kunn HadinotoWean Sin Cheow
Jan 25, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·María V Ramírez-RigoRuben H Manzo
Jun 9, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Nazareth Eliana CeschanMaría Verónica Ramírez-Rigo

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Citations

Mar 30, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Qun-Li LeiRan Ni

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