Influence of sample type and storage conditions on soluble CD40 ligand assessment

Clinical Chemistry
Michael WeberChristian W Hamm

Abstract

Several studies have consistently shown that soluble CD40 ligand (sCD40L) concentrations are increased in patients with acute coronary syndromes and can serve as a biomarker for risk stratification. However, few data are available on preanalytic conditions that impact sCD40L values. Thus, the aim of our prospective study was to evaluate the impact of sampling techniques and storage conditions on sCD40L concentrations. We included a total of 30 patients with no, stable, or unstable coronary heart disease. Blood samples were collected in gel-filled tubes without additives, in EDTA-filled tubes, and in citrate-filled tubes and were kept at various storage conditions. Median (interquartile range) sCD40L values at baseline were higher in serum samples [5.29 (3.89-6.33) microg/L] than in either EDTA plasma [0.78 (0.39-1.12) microg/L; P <0.001] or citrate plasma [0.37 (0.22-0.51) microg/L; P <0.001]. Serum values increased with delayed processing [7.94 (5.97-9.62) microg/L after 1.5 h (P <0.001) vs baseline; 10.55 (7.58-11.55) microg/L after 3 h (P <0.001) vs baseline]. However, after centrifugation, sCD40L values remained stable for all 3 sample types. Plasma, but not serum, samples are appropriate for sCD40L measurements. In general...Continue Reading

References

Mar 7, 2001·Cellular and Molecular Life Sciences : CMLS·U Schönbeck, P Libby
Mar 21, 2003·The New England Journal of Medicine·Christopher HeeschenUNKNOWN CAPTURE Study Investigators
Aug 13, 2003·Circulation·Nerea VaroUwe Schönbeck
Mar 19, 2005·Clinical Chemistry·Fred S AppleUNKNOWN Committee on Standardization of Markers of Cardiac Damage of the IFCC
May 26, 2005·Clinical Chemistry·Anna Margrét HalldórsdóttirCharles S Eby

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Citations

Mar 17, 2011·Critical Care : the Official Journal of the Critical Care Forum·Leonardo LorenteJosé A Páramo
Nov 29, 2007·Internal and Emergency Medicine·F SantilliG Davì
Nov 5, 2010·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Martin GrivaCestmir Cihalik
Oct 6, 2007·Journal of Thrombosis and Thrombolysis·Benjamin A OlenchockDavid A Morrow
Aug 20, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Eftihia SbarouniVassilis Voudris
Jan 26, 2016·Biopreservation and Biobanking·Jae-Eun LeeSo-Youn Shin
Mar 20, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Devin W KehlAlan S Maisel
Mar 26, 2009·Clinical Biochemistry·Barry McDonnellRichard O'Kennedy
Sep 28, 2012·Thrombosis and Haemostasis·Patrizia FerroniGiovanni Davì
Sep 6, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A H CharafeddineA D Kirk
May 25, 2010·Journal of Thrombosis and Haemostasis : JTH·P StarlingerC Brostjan
Mar 13, 2015·Experimental Hematology & Oncology·Antoine DewitteJean Ripoche
Aug 16, 2014·Thrombosis Research·Leonardo LorenteJuan M Borreguero-León
May 28, 2013·Free Radical Biology & Medicine·Julia SobierajskiTienush Rassaf
Apr 20, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·I Ramasamy
Sep 8, 2007·Dermatology : International Journal for Clinical and Investigative Dermatology·R WatanabeK Tamaki
Jul 9, 2021·Frontiers in Cardiovascular Medicine·Gaukhar BaidildinovaPaola E J van der Meijden
Oct 30, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Fabio AngeliClaudio Cavallini

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