Influence of selected formulation variables on the preparation of enzyme-entrapped Eudragit S100 microspheres

AAPS PharmSciTech
Manju RawatSwarnlata Saraf

Abstract

The aim of this work is to study the influence of formulation parameters in the preparation of sustained release enzyme-loaded Eudragit S100 microspheres by emulsion solvent diffusion technique. A 3(2) full factorial experiment was designed to study the effects of the amount of solvent (dichloromethane) and stabilizers (Tween 20, 40, or 80) on the drug content and microsphere size. The results of analysis of variance test for both effects indicated that the test is significant. The effect of amount of stabilizer was found to be higher on both responses (SS(Y1) = 45.60; SS(Y2) = 737.93), whereas solvent concentration comparatively produced significant effect on the size of microspheres (SS(Y1) = 0.81; SS(Y2) = 358.83). Scanning electron microscopy of microspheres with maximum drug content (2.5 mL dichloromethane and 0.1 mL Tween 80) demonstrated smooth surface spherical particles with mean diameter of 56.83 +/- 2.88 microm. The effect of formulation variables on the integrity of enzyme was confirmed by in vitro proteolytic activity. The enteric nature of microspheres was evaluated and results demonstrated ~6% to 7% release of enzyme in acidic medium. The release of enzyme from microspheres followed Higuchi kinetics. In phosphate...Continue Reading

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Citations

Oct 30, 2012·Journal of Materials Science. Materials in Medicine·Fu-Yin HsuChun-Chiang Huang
Jul 16, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Sandeep KumarMithu Maiti
Dec 8, 2010·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Santanu ChakrabortyKalyan Kumar Sen
Feb 26, 2009·Expert Opinion on Drug Delivery·Swarnlata Saraf
Jul 28, 2011·Expert Opinion on Drug Delivery·Bhupinder SinghNaveen Ahuja
Dec 26, 2012·Expert Opinion on Drug Delivery·Deependra SinghManju Rawat Singh
Sep 18, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Madhulika PradhanManju Rawat Singh
Nov 7, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Rajendra Jangde, Deependra Singh
Jun 2, 2009·International Journal of Pharmaceutics·Desiree KietzmannAlf Lamprecht
Aug 10, 2011·Journal of Microencapsulation·Lin ZhangGuangxi Zhai
Jan 1, 2014·Journal of Pharmaceutics·Ujwala A Shinde, Shivkumar S Kanojiya
Apr 3, 2020·AAPS PharmSciTech·María G BarreraClaudio J Salomón
May 22, 2020·International Journal of Pharmaceutics·Elie AkannyClaire Bordes

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