Abstract
Selenoprotein S (SelS), a transmembrane selenoprotein, may be related to the response of endoplasmic reticulum (ER) stress. In this report, the influence of selenite supplementation and SelS gene silence on beta-mercaptoethanol (beta-ME)-mediated ER stress and cell apoptosis in HepG2 cells were examined. The results showed that SelS protein expression was markedly increased by 10 mM beta-ME and 100 nM sodium selenite in HepG2 cells. GRP78 protein level was significantly increased after treatment with 10 mM beta-ME in HepG2 cells, which suggested that beta-ME was also an ER stress inducer. Meanwhile, beta-ME (10 mM) was found to induce cell apoptosis, which was alleviated obviously when cells were pretreated with 100 nM selenite before exposure to beta-ME. Moreover, the suppression of SelS gene by siRNA could aggravate HepG2 cell apoptosis induced by beta-ME significantly. In conclusion, these results suggested that beta-ME, also an ER stress agent, could induce cell apoptosis, and SelS may play an important role in protecting cells from apoptosis induced by ER stress in HepG2 cells.
Citations
Oct 4, 2011·Environmental Health Perspectives·Milan SrivastavaWilliam T Self
Feb 24, 2012·PloS One·Małgorzata DudkiewiczKrzysztof Pawłowski
Apr 25, 2013·PloS One·Jodi L BubenikDonna M Driscoll
Feb 12, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Dong YuShi-Wen Xu
Mar 13, 2012·Annual Review of Nutrition·Cindy D DavisJohn A Milner
Oct 2, 2014·Biological Trace Element Research·Haidong YaoShiwen Xu
Sep 19, 2015·Annals of Human Biology·Hong-Yun SunYu-Jing He
Dec 4, 2015·Biological Trace Element Research·Zijiang YangShu Li
Aug 1, 2015·Cell Stress & Chaperones·Pervez Ahmed KhosoShu Li
May 2, 2013·Oxidative Medicine and Cellular Longevity·Suzana MakpolWan Zurinah Wan Ngah
Nov 28, 2013·Free Radical Biology & Medicine·Bodo SpeckmannKum Kum Khanna
Apr 19, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alex B AddinsallNicole Stupka
Dec 18, 2018·Physiological Reports·Alex B AddinsallNicole Stupka
May 12, 2011·The Proceedings of the Nutrition Society·John Hesketh, Catherine Méplan
Aug 12, 2017·Cardiovascular Diabetology·Shan-Shan Yu, Jian-Ling Du
Feb 10, 2018·Current Medicinal Chemistry·Andrea SilvestriniAntonio Mancini
May 10, 2020·Antioxidants·Ying ZhangSeung-Rock Lee
May 29, 2018·Mediators of Inflammation·Siyuan CuiJianling Du
Jun 9, 2017·Mediators of Inflammation·Craig Robert WrightNicole Stupka
Jun 11, 2015·Journal of Cellular Biochemistry·Yali YeHongmei Liu
Mar 22, 2018·The Biochemical Journal·Alex B AddinsallNicole Stupka
Apr 26, 2016·Molecular Medicine Reports·Hong-Shuang QinYu-Xin Li
Oct 28, 2015·The Journal of Biological Chemistry·Jea Hwang LeeIck Young Kim
Sep 3, 2019·Free Radical Biology & Medicine·Andrea LeonardiThomas J Begley
Aug 28, 2020·Free Radical Biology & Medicine·Xu ZhangXin Gen Lei
Apr 10, 2013·Biochemistry·Jun LiuSharon Rozovsky
Aug 7, 2013·Biochemistry·Jun Liu, Sharon Rozovsky