Influence of SelS gene silence on beta-Mercaptoethanol-mediated endoplasmic reticulum stress and cell apoptosis in HepG2 cells

Biochimica Et Biophysica Acta
Shaoqing DuKaixun Huang

Abstract

Selenoprotein S (SelS), a transmembrane selenoprotein, may be related to the response of endoplasmic reticulum (ER) stress. In this report, the influence of selenite supplementation and SelS gene silence on beta-mercaptoethanol (beta-ME)-mediated ER stress and cell apoptosis in HepG2 cells were examined. The results showed that SelS protein expression was markedly increased by 10 mM beta-ME and 100 nM sodium selenite in HepG2 cells. GRP78 protein level was significantly increased after treatment with 10 mM beta-ME in HepG2 cells, which suggested that beta-ME was also an ER stress inducer. Meanwhile, beta-ME (10 mM) was found to induce cell apoptosis, which was alleviated obviously when cells were pretreated with 100 nM selenite before exposure to beta-ME. Moreover, the suppression of SelS gene by siRNA could aggravate HepG2 cell apoptosis induced by beta-ME significantly. In conclusion, these results suggested that beta-ME, also an ER stress agent, could induce cell apoptosis, and SelS may play an important role in protecting cells from apoptosis induced by ER stress in HepG2 cells.

Citations

Oct 4, 2011·Environmental Health Perspectives·Milan SrivastavaWilliam T Self
Feb 24, 2012·PloS One·Małgorzata DudkiewiczKrzysztof Pawłowski
Feb 12, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Dong YuShi-Wen Xu
Mar 13, 2012·Annual Review of Nutrition·Cindy D DavisJohn A Milner
May 2, 2013·Oxidative Medicine and Cellular Longevity·Suzana MakpolWan Zurinah Wan Ngah
Nov 28, 2013·Free Radical Biology & Medicine·Bodo SpeckmannKum Kum Khanna
Apr 19, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alex B AddinsallNicole Stupka
May 12, 2011·The Proceedings of the Nutrition Society·John Hesketh, Catherine Méplan
Aug 12, 2017·Cardiovascular Diabetology·Shan-Shan Yu, Jian-Ling Du
Feb 10, 2018·Current Medicinal Chemistry·Andrea SilvestriniAntonio Mancini
May 29, 2018·Mediators of Inflammation·Siyuan CuiJianling Du
Apr 26, 2016·Molecular Medicine Reports·Hong-Shuang QinYu-Xin Li
Aug 7, 2013·Biochemistry·Jun Liu, Sharon Rozovsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.