Influence of serum albumin and the flavonol quercetin on the peroxidase activity of metmyoglobin

Free Radical Biology & Medicine
Bénédicte LorrainOlivier Dangles

Abstract

Metmyoglobin (MbFe(III)), a major form of dietary iron, is an efficient inducer of lipid and protein oxidation. Indeed, MbFe(III) is able to cleave hydrogen peroxide and lipid hydroperoxides with subsequent formation of ferrylmyoglobin (MbFe(IV)=O) and lipid oxyl and peroxyl radicals. In the first part of this work, the mechanism of the reaction between MbFe(III) and H(2)O(2) is revisited with an emphasis on the influence of bovine serum albumin (BSA). BSA does not affect the rate of MbFe(IV)=O formation but inhibits the formation of a redox-inactive green pigment (heme-protein cross-link species). Although tightly bound to BSA, the flavonol quercetin is still able to reduce MbFe(IV)=O as a likely result of long-range electron transfers within a protein-protein complex. In the second part, BSA is shown to strongly slow down the metmyoglobin-catalyzed consumption of linoleic acid hydroperoxides with formation of ketones as the main products. In the process, only low concentrations of ferrylmyoglobin are slowly accumulated. A catalytic mechanism is proposed that involves a one-electron-oxidized metmyoglobin species distinct from ferrylmyoglobin.

References

Jul 7, 1995·The Journal of Biological Chemistry·M R GuntherR P Mason
Feb 26, 2000·Journal of Agricultural and Food Chemistry·C U CarlsenL H Skibsted
May 20, 2000·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·R A Tschirret-GuthP R Ortiz de Montellano
Jan 16, 2002·Current Opinion in Lipidology·Jeffrey S Cohn
May 2, 2002·Journal of Agricultural and Food Chemistry·Min Hu, Leif H Skibsted
Jun 27, 2002·Journal of Agricultural and Food Chemistry·Caroline P Baron, Henrik J Andersen
May 9, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Viviane TyssandierPatrick Borel
Aug 2, 2003·Archives of Biochemistry and Biophysics·Maiken V Kröger-OhlsenMogens L Andersen
Apr 28, 2005·Journal of Agricultural and Food Chemistry·Tair LapidotJoseph Kanner
Jan 25, 2008·Free Radical Biology & Medicine·Brandon J ReederMichael T Wilson
Sep 26, 2008·Journal of Agricultural and Food Chemistry·Ricard BouEric A Decker
Oct 31, 2008·Organic & Biomolecular Chemistry·Stéphanie GallandOlivier Dangles
Nov 17, 2009·Journal of Agricultural and Food Chemistry·Bénédicte LorrainClaire Dufour

❮ Previous
Next ❯

Citations

May 12, 2011·Journal of Agricultural and Food Chemistry·Silvia Helena LibardiDaniel R Cardoso
Aug 7, 2012·Journal of Agricultural and Food Chemistry·Bénédicte LorrainClaire Dufour
Nov 13, 2013·Journal of Asian Natural Products Research·Nai-Hao LuYi-Yuan Peng
Nov 25, 2011·Journal of Photochemistry and Photobiology. B, Biology·Fei DingLi Zhang
Feb 11, 2014·Journal of Agricultural and Food Chemistry·Silvia H LibardiDaniel R Cardoso
May 23, 2020·Journal of Inorganic Biochemistry·Lidia Gebicka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.