Influence of serum and hormones on bovine oocyte maturation and fertilization in vitro

Gamete Research
A I YounisR A Fayrer-Hosken

Abstract

Three approaches were investigated for improvement of in vitro maturation (IVM), in vitro fertilization (IVF), and early embryonic development in cattle. These were: 1) Selection of oocytes, 2) medium supplementation with fetal calf serum (FCS) and cow sera (D0, D1, D10, and D20 to correspond with estrus, metestrus, diestrus, and proestrus, respectively), and 3) addition of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol-17 beta (E2) during maturation. Greater proportions (percentage) of oocytes initially selected for their compact cumulus cells completed IVM and IVF when compared to unselected oocytes (P less than .05). Proportions (percentage) of selected oocytes that matured and cleaved after in vitro insemination according to serum type used for IVM were: FCS: 110/175 (62.9%) and 37/110 (33.6%) and D0: 130/145 (89.7%) and 52/130 (40.0%); D1 127/130 (97.7%) and 41/127 (32.3%); D10 95/98 (96.9%) and 35/95 (36.8%); D20:113/116 (97.4%) and 49/113 (43.4%). A higher proportion (P less than .05) of embryos resulting from the D20 group reached four- and eight-cell stages. In FCS-supplemented maturation media with no hormones added during maturation (control), results of IVM and IVF were 157/265 (59.2%) a...Continue Reading

References

Jan 1, 1979·Journal of Animal Science·L Leibfried, N L First
Feb 1, 1975·Journal of Reproduction and Fertility·I H Bae, R H Foote
Aug 1, 1988·Gamete Research·C L KeeferB G Brackett
Nov 1, 1987·Journal of Reproduction and Fertility·K P XuP Hyttel
Nov 1, 1984·Journal of Dairy Science·G D BallN L First
Apr 1, 1983·Biology of Reproduction·G D BallN L First
Aug 1, 1982·Biology of Reproduction·B G BrackettM A Dressel
Aug 1, 1980·Biology of Reproduction·B G BrackettW J Donawick
Jan 1, 1981·Theriogenology·R D DahlhausenT M Ludwick
Apr 1, 1986·Theriogenology·J J ParrishN L First
Oct 31, 1935·The Journal of Experimental Medicine·G Pincus, E V Enzmann

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Citations

Apr 1, 1996·Molecular Reproduction and Development·G C LuvoniB G Brackett
Mar 21, 1998·Molecular Reproduction and Development·S KölleG Palma
May 7, 1999·Molecular Reproduction and Development·R L Krisher, B D Bavister
Mar 1, 1990·Molecular Reproduction and Development·C L KeeferB G Brackett
Mar 1, 1991·Molecular Reproduction and Development·F de LoosT A Kruip
Nov 1, 1991·Molecular Reproduction and Development·G T O'NeillM H Kaufman
Dec 1, 1991·Molecular Reproduction and Development·K E WiemerG A Schultz
Dec 1, 1991·Molecular Reproduction and Development·P WillisR A Fayrer-Hosken
Feb 1, 1992·Molecular Reproduction and Development·A I Younis, B G Brackett
Mar 1, 1992·Molecular Reproduction and Development·F de LoosT A Kruip
Jan 1, 1993·Molecular Reproduction and Development·X YangR H Foote
Mar 1, 1993·Molecular Reproduction and Development·K E Mate, J C Rodger
Oct 1, 1993·Molecular Reproduction and Development·H Kato, A Iritani
Jan 1, 1994·Molecular Reproduction and Development·P WillisR A Fayrer-Hosken
Mar 1, 1994·Molecular Reproduction and Development·R J AsseyB Purwantara
Apr 1, 1994·Molecular Reproduction and Development·R D SchrammB D Bavister
Sep 1, 1994·Molecular Reproduction and Development·R J AsseyM Boland
Oct 1, 1994·Molecular Reproduction and Development·F A De LoosM M Bevers
Oct 1, 1992·Journal of Assisted Reproduction and Genetics·J SantalóA M Estop
Oct 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F GandolfiP Pocar
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A V Sirotkin
May 17, 2001·Theriogenology·T L Gable, G L Woods
Sep 27, 2001·Theriogenology·Y H ChoiE L Squire
May 7, 2002·Theriogenology·Margot Alves Nunes Dode, Charles Graves

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