Influence of short-term changes in dietary sulfur on the relative abundances of intestinal sulfate-reducing bacteria

Gut Microbes
Allison Dostal WebsterAlexander Khoruts

Abstract

High-protein diets may be linked to gut inflammation due to increased production of hydrogen sulfide (H2S), a potential toxin, as an end product of microbial fermentation in the colon by sulfidogenic sulfate-reducing bacteria (SRB). We hypothesized that dietary content of sulfur-containing amino acids (SAA) leads to variation in the relative abundances of intestinal SRB, which include Desulfovibrio and Bilophila taxa. To test this hypothesis we performed a pilot crossover study in four healthy volunteers, who consumed two interventional diets for 10-14 days, containing high or low SAA content. The total energy intake was similar between the two dietary extremes. Microbial communities were characterized by 16S rRNA gene amplicon and shotgun next-generation DNA sequencing. While the relative abundance of Desulfovibrio differed among participants (ANOVA P= 0.001), we could not detect a change with dietary treatments. Similarly, no differences in Bilophila abundance were observed among individuals or dietary arms. Inter-personal differences in microbial community composition and functional gene categories differed between subjects and these differences were maintained over the course of the study. These observations are consistent ...Continue Reading

References

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Citations

Apr 28, 2019·Nutrients·Levi M TeigenAlexander Khoruts
Sep 22, 2019·Nature Reviews. Microbiology·Aleksandra A KolodziejczykEran Elinav
Jan 8, 2021·BMC Bioinformatics·Nick W SmithWarren C McNabb
May 27, 2021·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Pabitra SahuRakesh K Tandon
Sep 28, 2021·Frontiers in Cell and Developmental Biology·Qingfeng WangPeizeng Yang
Oct 7, 2021·The Biochemical Journal·Stephen E WilkieColin Selman
Nov 13, 2021·JAMA Network Open·Yiqing WangAndrew T Chan
Jan 1, 2022·Experimental and Therapeutic Medicine·Magdalena DumitrescuAndra-Iulia Suceveanu

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Datasets Mentioned

BETA
SRP002424

Methods Mentioned

BETA
amplicon sequencing

Software Mentioned

NDSR
mothur
join
fastq
MetaPhlAn2
ANOSIM
HUMAnN2
UCHIME

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