Influence of soil fumigation by methyl bromide and methyl iodide on rhizosphere and phyllosphere microbial community structure

Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
A M IbekweC-H Yang

Abstract

Rhizosphere and phyllosphere microbial communities were evaluated on roots and leaves of growth chamber-grown lettuce (Lactuca sativa (L.) cv. Green Forest) plants by culture-dependent and -independent methods after soil fumigation. Denaturing gradient gel electrophoresis (DGGE) with 16S rRNA primers followed by cloning and sequencing was used to identify major rRNA bands from the rhizosphere and phyllosphere. Three weeks after fumigation, there were no differences (P = 0.16) in rhizosphere microbial communities between the fumigated treatments and the control. The same effect was observed during week seven after fumigation (P=0.49). Also, no significant differences (P=0.49) were found in the phyllosphere microbial communities between the fumigated treatments and the control during the growth period of the plant. A majority of the bands in the rhizosphere were related to known bacterial sequences with a 96 to 100 % sequence similarity. Some of the derived sequences were related to Pseudomonas syringae pv. tomato DC300 and Bradyrhizobium japonicum USDA 110. A total of 23 isolates were identified from leaf surface by both culture-dependent and independent methods, and only Photorhabdus luminescens was found on leaf surface using ...Continue Reading

Citations

Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S S Hirano, C D Upper
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C-H YangN T Keen
Jun 27, 2001·Applied and Environmental Microbiology·A M IbekweD E Crowley
Apr 26, 2002·Environmental Microbiology·Joel V Gagliardi, Jeffrey S Karns
Apr 2, 2003·Reviews of Environmental Contamination and Toxicology·Scott R YatesS K Papiernik
Dec 14, 2005·Applied and Environmental Microbiology·K SmallaL Forney
May 1, 2004·FEMS Microbiology Ecology·A M Ibekwe, C M Grieve
Oct 22, 2009·Letters in Applied Microbiology·A Mark IbekweC-H Yang

Related Concepts

Lactuca sativa
Rhizosphere
Lactuca sativa antigen
SDS-PAGE
Photorhabdus luminescens
Marchantiae
Seasonal Variation
Ribosomal RNA
Sequencing
Bradyrhizobium japonicum

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
C-H YangN T Keen
Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
A M IbekweC-H Yang
© 2020 Meta ULC. All rights reserved