Influence of soil fumigation by methyl bromide and methyl iodide on rhizosphere and phyllosphere microbial community structure

Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
A M IbekweC-H Yang


Rhizosphere and phyllosphere microbial communities were evaluated on roots and leaves of growth chamber-grown lettuce (Lactuca sativa (L.) cv. Green Forest) plants by culture-dependent and -independent methods after soil fumigation. Denaturing gradient gel electrophoresis (DGGE) with 16S rRNA primers followed by cloning and sequencing was used to identify major rRNA bands from the rhizosphere and phyllosphere. Three weeks after fumigation, there were no differences (P = 0.16) in rhizosphere microbial communities between the fumigated treatments and the control. The same effect was observed during week seven after fumigation (P=0.49). Also, no significant differences (P=0.49) were found in the phyllosphere microbial communities between the fumigated treatments and the control during the growth period of the plant. A majority of the bands in the rhizosphere were related to known bacterial sequences with a 96 to 100 % sequence similarity. Some of the derived sequences were related to Pseudomonas syringae pv. tomato DC300 and Bradyrhizobium japonicum USDA 110. A total of 23 isolates were identified from leaf surface by both culture-dependent and independent methods, and only Photorhabdus luminescens was found on leaf surface using ...Continue Reading


Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S S Hirano, C D Upper
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·C-H YangN T Keen
Jun 27, 2001·Applied and Environmental Microbiology·A M IbekweD E Crowley
Apr 26, 2002·Environmental Microbiology·Joel V Gagliardi, Jeffrey S Karns
Apr 2, 2003·Reviews of Environmental Contamination and Toxicology·Scott R YatesS K Papiernik
Dec 14, 2005·Applied and Environmental Microbiology·K SmallaL Forney
May 1, 2004·FEMS Microbiology Ecology·A M Ibekwe, C M Grieve
Oct 22, 2009·Letters in Applied Microbiology·A Mark IbekweC-H Yang

Related Concepts

Lactuca sativa
Lactuca sativa antigen
Photorhabdus luminescens
Seasonal Variation
Ribosomal RNA
Bradyrhizobium japonicum

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Related Papers

Proceedings of the National Academy of Sciences of the United States of America
C-H YangN T Keen
Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
A M IbekweC-H Yang
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