Influence of soluble environmental factors on the development of fetal brain acetylcholinesterase-positive neurons cultured in a chemically defined medium: comparison with the effects of L-triiodothyronine (L-T3)

Brain Research. Developmental Brain Research
R GarzaJ H Dussault

Abstract

In cerebral hemisphere neuronal cultures derived from 15-day-old rat embryos, the addition of L-triiodothyronine (L-T3) or nerve growth factor (NGF) enhanced the expression of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities in a dose-dependent manner. When cultures were supplemented with both agents at maximal effective concentrations, the stimulation in ChAT and AChE activities was significantly greater than the sum of the individual effects. Conversely, when the cultures were exposed to astrocyte conditioned medium grown in the presence or absence of L-T3 (CM + L-T3 or CM-L-T3). laminin and fibroblast growth factor (FGF), ChAT and AChE activities were not stimulated above those of control cultures when added alone or in combination with L-T3. Furthermore, L-T3, NGF, CMs, laminin and FGF did not affect AChE+ cell survival, but significantly increased neurite outgrowth and branching with NGF and L-T3 being the most powerful agents followed by CMs, laminin and FGF. Additionally, the simultaneous addition of L-T3 with either laminin or FGF in culture, caused an additive effect of L-T2 in the neurite density of AChE+ cells with both agents. This study shows that (1) thyroid hormones do not act through t...Continue Reading

References

Jan 1, 1986·Journal of Neuroscience Research·S K Pixley, C W Cotman
Apr 14, 1986·Biochemical and Biophysical Research Communications·S FurukawaK Hayashi
Mar 1, 1989·Brain Research. Developmental Brain Research·M LuoJ H Dussault
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G W VasiosL J Gudas
Aug 1, 1987·Endocrine Reviews·J H OppenheimerH C Freake
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P WalickeR Guillemin
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R S MorrisonR A Bradshaw
Jul 24, 1986·Neuroscience Letters·B PettmannM Sensenbrenner
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A D LanderL F Reichardt
Sep 1, 1983·Brain Research·R S Morrison, J de Vellis
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H W MüllerW Seifert
Jun 15, 1980·Experientia·M SensenbrennerB J Meyer
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jan 1, 1985·Neurochemistry International·J Puymirat

❮ Previous
Next ❯

Citations

Dec 1, 1992·Brain Research. Molecular Brain Research·T GiordanoS P Arneric
Dec 1, 1993·Progress in Neurobiology·M Sensenbrenner
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B R SouthwellG Schreiber
Feb 12, 2002·Neuroscience and Biobehavioral Reviews·Jeremy W SmithJames W Smythe
Dec 1, 1992·Neurochemical Research·A CestelliI Di Liegro
Sep 22, 2009·Brain Research·Alfonso Carreón-RodríguezLeonor Pérez-Martínez
Jan 1, 1995·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·J S HanT Cook
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity·Allan J WilliamsGraham R Williams

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.