Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula.

BioTechniques
Georg SchützLea Atanasova

Abstract

Species of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploited as biocontrol agents. A careful preparation of Trichoderma inocula, which consists mainly of conidia (asexual spores), is of immense importance. Conidia concentration is still mostly determined with the help of a hemocytometer; however, as a more accurate and time-saving alternative, absorbance can be used to estimate fungal spore counts. We established a spectrophotometric method for fast and reliable preparation of Trichoderma inocula by evaluating the effect of size, shape and pigmentation of the conidia at different wavelengths.

References

Aug 19, 1961·Biochimica Et Biophysica Acta·A L KOCH
Nov 15, 2011·Applied Microbiology and Biotechnology·Fatma UzbasBernhard Seiboth
Nov 15, 2013·Nucleic Acids Research·Henrik NordbergInna Dubchak

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Citations

Jun 13, 2021·Enzyme and Microbial Technology·Florian CsarmanRoland Ludwig

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Methods Mentioned

BETA
infrared reflectance

Software Mentioned

R
RStudio

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