Influence of staining and sampling procedures on goat sperm morphometry using the Sperm Class Analyzer

Theriogenology
M HidalgoJ Dorado

Abstract

Computer-assisted sperm morphometry analysis (ASMA) has improved the assessment of sperm morphology, but the results depend on the use of adequate sampling and staining procedures of spermatozoa from individual species. In this study, the Sperm Class Analyzer ASMA system was used for the morphometric analysis of goat sperm heads. Semen samples, obtained from four bucks, were used to evaluate the influence of three staining procedures (Diff-Quik, Hemacolor and Harris' Haematoxylin) on the accuracy of image processing and sperm morphometry, the effect of the number of cells analysed and the repeatability of the method. These experiments were performed to obtain objective, accurate and reliable sperm morphometric measurements of goat spermatozoa. Diff-Quik and Harris' Haematoxylin were significantly (p<0.05) more accurate than Hemacolor. However, Diff-Quik obtained the highest proportion of correctly analysed sperm heads (86.06%) and the lowest coefficients of variation on the image processing and morphometric measurements. The staining methods affected significantly the sperm dimensions (p<0.001) with increased values from Diff-Quik than Hemacolor and Harris' Haematoxylin, respectively (Diff-Quik>Hemacolor>Harris' Haematoxylin). ...Continue Reading

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Citations

Nov 29, 2013·Animal : an International Journal of Animal Bioscience·I OrtizM Hidalgo
Jul 3, 2007·Fertility and Sterility·Ralf HenkelRoelof Menkveld
Jul 14, 2007·Animal Reproduction Science·Manuel HidalgoCarles Soler
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May 20, 2021·Molecular Reproduction and Development·Ester TeránSebastián Demyda-Peyrás

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